Cargando…

Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium

Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant host adaptations are currently unknown. We sequenced the genome of V. dahliae Vd991, which is highly virulent on its original host, cotton, and performed compari...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Jie‐Yin, Liu, Chun, Gui, Yue‐Jing, Si, Kai‐Wei, Zhang, Dan‐Dan, Wang, Jie, Short, Dylan P. G., Huang, Jin‐Qun, Li, Nan‐Yang, Liang, Yong, Zhang, Wen‐Qi, Yang, Lin, Ma, Xue‐Feng, Li, Ting‐Gang, Zhou, Lei, Wang, Bao‐Li, Bao, Yu‐Ming, Subbarao, Krishna V., Zhang, Geng‐Yun, Dai, Xiao‐Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765495/
https://www.ncbi.nlm.nih.gov/pubmed/29084346
http://dx.doi.org/10.1111/nph.14861
_version_ 1783292242687950848
author Chen, Jie‐Yin
Liu, Chun
Gui, Yue‐Jing
Si, Kai‐Wei
Zhang, Dan‐Dan
Wang, Jie
Short, Dylan P. G.
Huang, Jin‐Qun
Li, Nan‐Yang
Liang, Yong
Zhang, Wen‐Qi
Yang, Lin
Ma, Xue‐Feng
Li, Ting‐Gang
Zhou, Lei
Wang, Bao‐Li
Bao, Yu‐Ming
Subbarao, Krishna V.
Zhang, Geng‐Yun
Dai, Xiao‐Feng
author_facet Chen, Jie‐Yin
Liu, Chun
Gui, Yue‐Jing
Si, Kai‐Wei
Zhang, Dan‐Dan
Wang, Jie
Short, Dylan P. G.
Huang, Jin‐Qun
Li, Nan‐Yang
Liang, Yong
Zhang, Wen‐Qi
Yang, Lin
Ma, Xue‐Feng
Li, Ting‐Gang
Zhou, Lei
Wang, Bao‐Li
Bao, Yu‐Ming
Subbarao, Krishna V.
Zhang, Geng‐Yun
Dai, Xiao‐Feng
author_sort Chen, Jie‐Yin
collection PubMed
description Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant host adaptations are currently unknown. We sequenced the genome of V. dahliae Vd991, which is highly virulent on its original host, cotton, and performed comparisons with the reference genomes of JR2 (from tomato) and VdLs.17 (from lettuce). Pathogenicity‐related factor prediction, orthology and multigene family classification, transcriptome analyses, phylogenetic analyses, and pathogenicity experiments were performed. The Vd991 genome harbored several exclusive, lineage‐specific (LS) genes within LS regions (LSRs). Deletion mutants of the seven genes within one LSR (G‐LSR2) in Vd991 were less virulent only on cotton. Integration of G‐LSR2 genes individually into JR2 and VdLs.17 resulted in significantly enhanced virulence on cotton but did not affect virulence on tomato or lettuce. Transcription levels of the seven LS genes in Vd991 were higher during the early stages of cotton infection, as compared with other hosts. Phylogenetic analyses suggested that G‐LSR2 was acquired from Fusarium oxysporum f. sp. vasinfectum through horizontal gene transfer. Our results provide evidence that horizontal gene transfer from Fusarium to Vd991 contributed significantly to its adaptation to cotton and may represent a significant mechanism in the evolution of an asexual plant pathogen.
format Online
Article
Text
id pubmed-5765495
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-57654952018-02-01 Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium Chen, Jie‐Yin Liu, Chun Gui, Yue‐Jing Si, Kai‐Wei Zhang, Dan‐Dan Wang, Jie Short, Dylan P. G. Huang, Jin‐Qun Li, Nan‐Yang Liang, Yong Zhang, Wen‐Qi Yang, Lin Ma, Xue‐Feng Li, Ting‐Gang Zhou, Lei Wang, Bao‐Li Bao, Yu‐Ming Subbarao, Krishna V. Zhang, Geng‐Yun Dai, Xiao‐Feng New Phytol Research Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant host adaptations are currently unknown. We sequenced the genome of V. dahliae Vd991, which is highly virulent on its original host, cotton, and performed comparisons with the reference genomes of JR2 (from tomato) and VdLs.17 (from lettuce). Pathogenicity‐related factor prediction, orthology and multigene family classification, transcriptome analyses, phylogenetic analyses, and pathogenicity experiments were performed. The Vd991 genome harbored several exclusive, lineage‐specific (LS) genes within LS regions (LSRs). Deletion mutants of the seven genes within one LSR (G‐LSR2) in Vd991 were less virulent only on cotton. Integration of G‐LSR2 genes individually into JR2 and VdLs.17 resulted in significantly enhanced virulence on cotton but did not affect virulence on tomato or lettuce. Transcription levels of the seven LS genes in Vd991 were higher during the early stages of cotton infection, as compared with other hosts. Phylogenetic analyses suggested that G‐LSR2 was acquired from Fusarium oxysporum f. sp. vasinfectum through horizontal gene transfer. Our results provide evidence that horizontal gene transfer from Fusarium to Vd991 contributed significantly to its adaptation to cotton and may represent a significant mechanism in the evolution of an asexual plant pathogen. John Wiley and Sons Inc. 2017-10-30 2018-01 /pmc/articles/PMC5765495/ /pubmed/29084346 http://dx.doi.org/10.1111/nph.14861 Text en © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Chen, Jie‐Yin
Liu, Chun
Gui, Yue‐Jing
Si, Kai‐Wei
Zhang, Dan‐Dan
Wang, Jie
Short, Dylan P. G.
Huang, Jin‐Qun
Li, Nan‐Yang
Liang, Yong
Zhang, Wen‐Qi
Yang, Lin
Ma, Xue‐Feng
Li, Ting‐Gang
Zhou, Lei
Wang, Bao‐Li
Bao, Yu‐Ming
Subbarao, Krishna V.
Zhang, Geng‐Yun
Dai, Xiao‐Feng
Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium
title Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium
title_full Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium
title_fullStr Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium
title_full_unstemmed Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium
title_short Comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium
title_sort comparative genomics reveals cotton‐specific virulence factors in flexible genomic regions in verticillium dahliae and evidence of horizontal gene transfer from fusarium
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765495/
https://www.ncbi.nlm.nih.gov/pubmed/29084346
http://dx.doi.org/10.1111/nph.14861
work_keys_str_mv AT chenjieyin comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT liuchun comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT guiyuejing comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT sikaiwei comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT zhangdandan comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT wangjie comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT shortdylanpg comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT huangjinqun comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT linanyang comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT liangyong comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT zhangwenqi comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT yanglin comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT maxuefeng comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT litinggang comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT zhoulei comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT wangbaoli comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT baoyuming comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT subbaraokrishnav comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT zhanggengyun comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium
AT daixiaofeng comparativegenomicsrevealscottonspecificvirulencefactorsinflexiblegenomicregionsinverticilliumdahliaeandevidenceofhorizontalgenetransferfromfusarium