Cargando…
Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes
Preterm premature rupture of membranes (pPROM) is a major cause of preterm birth. Recently, extracellular matrix-directed treatment is applied for wound healing. Here, we used a pregnant mouse model to test the efficacy of collagen type 1 gel for healing of the prematurely ruptured fetal membranes....
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766504/ https://www.ncbi.nlm.nih.gov/pubmed/29330408 http://dx.doi.org/10.1038/s41598-017-18787-9 |
_version_ | 1783292363348639744 |
---|---|
author | Mogami, Haruta Kishore, Annavarapu Hari Word, R. Ann |
author_facet | Mogami, Haruta Kishore, Annavarapu Hari Word, R. Ann |
author_sort | Mogami, Haruta |
collection | PubMed |
description | Preterm premature rupture of membranes (pPROM) is a major cause of preterm birth. Recently, extracellular matrix-directed treatment is applied for wound healing. Here, we used a pregnant mouse model to test the efficacy of collagen type 1 gel for healing of the prematurely ruptured fetal membranes. Although injection of PBS into the ruptured fetal membranes resulted in 40% closure, injection of collagen type 1 improved closure rates to 90% within 72 h. Macrophages of the M2 wound healing phenotype were entrapped in the collagen layer. In primary human amnion mesenchymal cells, collagen type 1 gels activated collagen receptor discoidin domain receptor 2 (DDR2) to induce myosin light chain phosphorylation and migration of injured amnion mesenchymal cells. These findings define the mechanisms for matrix-directed therapeutics for pPROM. |
format | Online Article Text |
id | pubmed-5766504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57665042018-01-17 Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes Mogami, Haruta Kishore, Annavarapu Hari Word, R. Ann Sci Rep Article Preterm premature rupture of membranes (pPROM) is a major cause of preterm birth. Recently, extracellular matrix-directed treatment is applied for wound healing. Here, we used a pregnant mouse model to test the efficacy of collagen type 1 gel for healing of the prematurely ruptured fetal membranes. Although injection of PBS into the ruptured fetal membranes resulted in 40% closure, injection of collagen type 1 improved closure rates to 90% within 72 h. Macrophages of the M2 wound healing phenotype were entrapped in the collagen layer. In primary human amnion mesenchymal cells, collagen type 1 gels activated collagen receptor discoidin domain receptor 2 (DDR2) to induce myosin light chain phosphorylation and migration of injured amnion mesenchymal cells. These findings define the mechanisms for matrix-directed therapeutics for pPROM. Nature Publishing Group UK 2018-01-12 /pmc/articles/PMC5766504/ /pubmed/29330408 http://dx.doi.org/10.1038/s41598-017-18787-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mogami, Haruta Kishore, Annavarapu Hari Word, R. Ann Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes |
title | Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes |
title_full | Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes |
title_fullStr | Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes |
title_full_unstemmed | Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes |
title_short | Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes |
title_sort | collagen type 1 accelerates healing of ruptured fetal membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766504/ https://www.ncbi.nlm.nih.gov/pubmed/29330408 http://dx.doi.org/10.1038/s41598-017-18787-9 |
work_keys_str_mv | AT mogamiharuta collagentype1accelerateshealingofrupturedfetalmembranes AT kishoreannavarapuhari collagentype1accelerateshealingofrupturedfetalmembranes AT wordrann collagentype1accelerateshealingofrupturedfetalmembranes |