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Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes

Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the “inputs” and “outputs,” remains a major challenge. Here, we present the development o...

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Autores principales: Schwarz, D., Kollo, M., Bosch, C., Feinauer, C., Whiteley, I., Margrie, T. W., Cutforth, T., Schaefer, A. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766516/
https://www.ncbi.nlm.nih.gov/pubmed/29330458
http://dx.doi.org/10.1038/s41467-017-02560-7
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author Schwarz, D.
Kollo, M.
Bosch, C.
Feinauer, C.
Whiteley, I.
Margrie, T. W.
Cutforth, T.
Schaefer, A. T.
author_facet Schwarz, D.
Kollo, M.
Bosch, C.
Feinauer, C.
Whiteley, I.
Margrie, T. W.
Cutforth, T.
Schaefer, A. T.
author_sort Schwarz, D.
collection PubMed
description Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the “inputs” and “outputs,” remains a major challenge. Here, we present the development of nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation of a substantial volume of neuronal tissue. Combining finite element modeling and focused ion beam milling, NEMs permit substantially higher stimulation intensities compared to conventional glass capillaries, allowing for larger volumes configurable to the geometry of the target circuit. We apply NEMs to achieve near-complete labeling of the neuronal network associated with a genetically identified olfactory glomerulus. This allows us to detect sparse higher-order features of the wiring architecture that are inaccessible to statistical labeling approaches. Thus, NEM labeling provides crucial complementary information to dense circuit reconstruction techniques. Relying solely on targeting an electrode to the region of interest and passive biophysical properties largely common across cell types, this can easily be employed anywhere in the CNS.
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spelling pubmed-57665162018-01-18 Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes Schwarz, D. Kollo, M. Bosch, C. Feinauer, C. Whiteley, I. Margrie, T. W. Cutforth, T. Schaefer, A. T. Nat Commun Article Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the “inputs” and “outputs,” remains a major challenge. Here, we present the development of nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation of a substantial volume of neuronal tissue. Combining finite element modeling and focused ion beam milling, NEMs permit substantially higher stimulation intensities compared to conventional glass capillaries, allowing for larger volumes configurable to the geometry of the target circuit. We apply NEMs to achieve near-complete labeling of the neuronal network associated with a genetically identified olfactory glomerulus. This allows us to detect sparse higher-order features of the wiring architecture that are inaccessible to statistical labeling approaches. Thus, NEM labeling provides crucial complementary information to dense circuit reconstruction techniques. Relying solely on targeting an electrode to the region of interest and passive biophysical properties largely common across cell types, this can easily be employed anywhere in the CNS. Nature Publishing Group UK 2018-01-12 /pmc/articles/PMC5766516/ /pubmed/29330458 http://dx.doi.org/10.1038/s41467-017-02560-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schwarz, D.
Kollo, M.
Bosch, C.
Feinauer, C.
Whiteley, I.
Margrie, T. W.
Cutforth, T.
Schaefer, A. T.
Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
title Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
title_full Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
title_fullStr Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
title_full_unstemmed Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
title_short Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
title_sort architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766516/
https://www.ncbi.nlm.nih.gov/pubmed/29330458
http://dx.doi.org/10.1038/s41467-017-02560-7
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