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Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus

Robotics is continuously being integrated in animal behaviour studies to create customizable, controllable, and repeatable stimuli. However, few systems have capitalized on recent breakthroughs in computer vision and real-time control to enable a two-way interaction between the animal and the robot....

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Detalles Bibliográficos
Autores principales: Kim, Changsu, Ruberto, Tommaso, Phamduy, Paul, Porfiri, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766612/
https://www.ncbi.nlm.nih.gov/pubmed/29330523
http://dx.doi.org/10.1038/s41598-017-19083-2
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author Kim, Changsu
Ruberto, Tommaso
Phamduy, Paul
Porfiri, Maurizio
author_facet Kim, Changsu
Ruberto, Tommaso
Phamduy, Paul
Porfiri, Maurizio
author_sort Kim, Changsu
collection PubMed
description Robotics is continuously being integrated in animal behaviour studies to create customizable, controllable, and repeatable stimuli. However, few systems have capitalized on recent breakthroughs in computer vision and real-time control to enable a two-way interaction between the animal and the robot. Here, we present a “closed-loop control” system to investigate the behaviour of zebrafish, a popular animal model in preclinical studies. The system allows for actuating a biologically-inspired 3D-printed replica in a 3D workspace, in response to the behaviour of a zebrafish. We demonstrate the role of closed-loop control in modulating the response of zebrafish, across a range of behavioural and information-theoretic measures. Our results suggest that closed-loop control could enhance the degree of biomimicry of the replica, by increasing the attraction of live subjects and their interaction with the stimulus. Interactive experiments hold promise to advance our understanding of zebrafish, offering new means for high throughput behavioural phenotyping.
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spelling pubmed-57666122018-01-25 Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus Kim, Changsu Ruberto, Tommaso Phamduy, Paul Porfiri, Maurizio Sci Rep Article Robotics is continuously being integrated in animal behaviour studies to create customizable, controllable, and repeatable stimuli. However, few systems have capitalized on recent breakthroughs in computer vision and real-time control to enable a two-way interaction between the animal and the robot. Here, we present a “closed-loop control” system to investigate the behaviour of zebrafish, a popular animal model in preclinical studies. The system allows for actuating a biologically-inspired 3D-printed replica in a 3D workspace, in response to the behaviour of a zebrafish. We demonstrate the role of closed-loop control in modulating the response of zebrafish, across a range of behavioural and information-theoretic measures. Our results suggest that closed-loop control could enhance the degree of biomimicry of the replica, by increasing the attraction of live subjects and their interaction with the stimulus. Interactive experiments hold promise to advance our understanding of zebrafish, offering new means for high throughput behavioural phenotyping. Nature Publishing Group UK 2018-01-12 /pmc/articles/PMC5766612/ /pubmed/29330523 http://dx.doi.org/10.1038/s41598-017-19083-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kim, Changsu
Ruberto, Tommaso
Phamduy, Paul
Porfiri, Maurizio
Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
title Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
title_full Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
title_fullStr Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
title_full_unstemmed Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
title_short Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
title_sort closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766612/
https://www.ncbi.nlm.nih.gov/pubmed/29330523
http://dx.doi.org/10.1038/s41598-017-19083-2
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