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Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis

OBJECTIVE: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the...

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Autores principales: Wang, Yixin, Jin, Long, Ma, Jideng, Chen, Li, Fu, Yuhua, Long, Keren, Hu, Silu, Song, Yang, Shang, Dazhi, Tang, Qianzi, Wang, Xun, Li, Xuewei, Li, Mingzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767500/
https://www.ncbi.nlm.nih.gov/pubmed/29059727
http://dx.doi.org/10.5713/ajas.17.0518
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author Wang, Yixin
Jin, Long
Ma, Jideng
Chen, Li
Fu, Yuhua
Long, Keren
Hu, Silu
Song, Yang
Shang, Dazhi
Tang, Qianzi
Wang, Xun
Li, Xuewei
Li, Mingzhou
author_facet Wang, Yixin
Jin, Long
Ma, Jideng
Chen, Li
Fu, Yuhua
Long, Keren
Hu, Silu
Song, Yang
Shang, Dazhi
Tang, Qianzi
Wang, Xun
Li, Xuewei
Li, Mingzhou
author_sort Wang, Yixin
collection PubMed
description OBJECTIVE: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. METHODS: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. RESULTS: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. CONCLUSION: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.
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spelling pubmed-57675002018-02-01 Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis Wang, Yixin Jin, Long Ma, Jideng Chen, Li Fu, Yuhua Long, Keren Hu, Silu Song, Yang Shang, Dazhi Tang, Qianzi Wang, Xun Li, Xuewei Li, Mingzhou Asian-Australas J Anim Sci Article OBJECTIVE: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. METHODS: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. RESULTS: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. CONCLUSION: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2018-02 2017-10-20 /pmc/articles/PMC5767500/ /pubmed/29059727 http://dx.doi.org/10.5713/ajas.17.0518 Text en Copyright © 2018 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Wang, Yixin
Jin, Long
Ma, Jideng
Chen, Li
Fu, Yuhua
Long, Keren
Hu, Silu
Song, Yang
Shang, Dazhi
Tang, Qianzi
Wang, Xun
Li, Xuewei
Li, Mingzhou
Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis
title Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis
title_full Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis
title_fullStr Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis
title_full_unstemmed Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis
title_short Hemicastration induced spermatogenesis-related DNA methylation and gene expression changes in mice testis
title_sort hemicastration induced spermatogenesis-related dna methylation and gene expression changes in mice testis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767500/
https://www.ncbi.nlm.nih.gov/pubmed/29059727
http://dx.doi.org/10.5713/ajas.17.0518
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