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Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats

BACKGROUND: Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial. OBJECTIVE: In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and hist...

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Autores principales: Salarkia, Ehsan, Sepehri, Gholamreza, Torabzadeh, Parvin, Abshenas, Jalil, Saberi, Arezoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Clinical Center for Infertility 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767643/
https://www.ncbi.nlm.nih.gov/pubmed/29387828
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author Salarkia, Ehsan
Sepehri, Gholamreza
Torabzadeh, Parvin
Abshenas, Jalil
Saberi, Arezoo
author_facet Salarkia, Ehsan
Sepehri, Gholamreza
Torabzadeh, Parvin
Abshenas, Jalil
Saberi, Arezoo
author_sort Salarkia, Ehsan
collection PubMed
description BACKGROUND: Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial. OBJECTIVE: In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and histological changes of testes in male rats. MATERIALS AND METHODS: In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i.p., and IV- IX received co-trimoxazole (30, 60, and 120 mg/kg/daily; i.p.)+folic acid (1 mg/kg/daily; i.p.) for 14 or 28 days. Sperm samples were obtained from each group at the end of 14(th) and 28(th) days. Sperm numbers, motility, and viability were evaluated on a hemocytometer. Hematoxylin and Eosin stained testes were done for evaluation ofthe number of Leydig cells, vascularity, spermatids, spermatocytes, and means of seminiferous tubules diameter under light microscopy. RESULTS: Co-trimoxazole treatment for either 14 or 28 days caused a significant decrease in the percentage of sperm number, motility, and viability (p<0.001) compared to the control group. Also, high doses of co-trimoxazole caused a significant decrease in testes structural abnormalities means of seminiferous tubules diameter, spermatids, and spermatogonia) compared to the vehicle group (p<0.001). Folic acid co-administration with co-trimoxazole partially reversed the decrease in sperm quality and structural abnormalities of high doses of co-trimoxazole (60 and 120 mg/kg/daily) (p<0.001). CONCLUSION: The data showed the adverse effects of co-trimoxazole on sperm quality and testes morphology which was protected partially by folic acid co-administration in rats. The underlying mechanism (s) needs further investigations.
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spelling pubmed-57676432018-01-31 Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats Salarkia, Ehsan Sepehri, Gholamreza Torabzadeh, Parvin Abshenas, Jalil Saberi, Arezoo Int J Reprod Biomed Original Article BACKGROUND: Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial. OBJECTIVE: In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and histological changes of testes in male rats. MATERIALS AND METHODS: In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i.p., and IV- IX received co-trimoxazole (30, 60, and 120 mg/kg/daily; i.p.)+folic acid (1 mg/kg/daily; i.p.) for 14 or 28 days. Sperm samples were obtained from each group at the end of 14(th) and 28(th) days. Sperm numbers, motility, and viability were evaluated on a hemocytometer. Hematoxylin and Eosin stained testes were done for evaluation ofthe number of Leydig cells, vascularity, spermatids, spermatocytes, and means of seminiferous tubules diameter under light microscopy. RESULTS: Co-trimoxazole treatment for either 14 or 28 days caused a significant decrease in the percentage of sperm number, motility, and viability (p<0.001) compared to the control group. Also, high doses of co-trimoxazole caused a significant decrease in testes structural abnormalities means of seminiferous tubules diameter, spermatids, and spermatogonia) compared to the vehicle group (p<0.001). Folic acid co-administration with co-trimoxazole partially reversed the decrease in sperm quality and structural abnormalities of high doses of co-trimoxazole (60 and 120 mg/kg/daily) (p<0.001). CONCLUSION: The data showed the adverse effects of co-trimoxazole on sperm quality and testes morphology which was protected partially by folic acid co-administration in rats. The underlying mechanism (s) needs further investigations. Research and Clinical Center for Infertility 2017-10 /pmc/articles/PMC5767643/ /pubmed/29387828 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Salarkia, Ehsan
Sepehri, Gholamreza
Torabzadeh, Parvin
Abshenas, Jalil
Saberi, Arezoo
Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
title Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
title_full Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
title_fullStr Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
title_full_unstemmed Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
title_short Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
title_sort effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767643/
https://www.ncbi.nlm.nih.gov/pubmed/29387828
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