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Evaluation of new technique of sterilization using biological indicator
BACKGROUND: A novel technique of sterilization of endodontic files is introduced in this article. AIMS: Newly introduced sterilization unit, named “SteriFast” is compared with autoclave and glass bead sterilizer using biological indicator. MATERIALS AND METHODS: Spore strips of Bacillus pumilus were...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767831/ https://www.ncbi.nlm.nih.gov/pubmed/29386784 http://dx.doi.org/10.4103/JCD.JCD_253_16 |
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author | Sheth, Nomal Chintan Rathod, Yogesh V. Shenoi, Pratima R. Shori, Deepa D. Khode, Rajiv T. Khadse, Amruta P. |
author_facet | Sheth, Nomal Chintan Rathod, Yogesh V. Shenoi, Pratima R. Shori, Deepa D. Khode, Rajiv T. Khadse, Amruta P. |
author_sort | Sheth, Nomal Chintan |
collection | PubMed |
description | BACKGROUND: A novel technique of sterilization of endodontic files is introduced in this article. AIMS: Newly introduced sterilization unit, named “SteriFast” is compared with autoclave and glass bead sterilizer using biological indicator. MATERIALS AND METHODS: Spore strips of Bacillus pumilus were cultured in nutrient broth. This cultured media was used to contaminate the experimental samples of endodontic files. These contaminated files were sterilized using three different techniques. The sterilized files were transferred into nutrient medium under aseptic condition. The results were observed after 24 h, 48 h, and 7 days. RESULTS: The results showed that autoclave and new sterilization device (SteriFast) showed complete sterilization. The files sterilized using glass bead sterilizer showed bacterial growth (80%). CONCLUSIONS: Thus, it proves that autoclave and SteriFast are ideal techniques of sterilization of endodontic files. Glass bead sterilizer does not completely sterilize the files. The article also compares SteriFast and autoclave in other aspects such as its design, basic principle, advantages, and disadvantages. The article also describes features and design of SteriFast, used for all kind of small dental instruments. |
format | Online Article Text |
id | pubmed-5767831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57678312018-01-31 Evaluation of new technique of sterilization using biological indicator Sheth, Nomal Chintan Rathod, Yogesh V. Shenoi, Pratima R. Shori, Deepa D. Khode, Rajiv T. Khadse, Amruta P. J Conserv Dent Original Research Article BACKGROUND: A novel technique of sterilization of endodontic files is introduced in this article. AIMS: Newly introduced sterilization unit, named “SteriFast” is compared with autoclave and glass bead sterilizer using biological indicator. MATERIALS AND METHODS: Spore strips of Bacillus pumilus were cultured in nutrient broth. This cultured media was used to contaminate the experimental samples of endodontic files. These contaminated files were sterilized using three different techniques. The sterilized files were transferred into nutrient medium under aseptic condition. The results were observed after 24 h, 48 h, and 7 days. RESULTS: The results showed that autoclave and new sterilization device (SteriFast) showed complete sterilization. The files sterilized using glass bead sterilizer showed bacterial growth (80%). CONCLUSIONS: Thus, it proves that autoclave and SteriFast are ideal techniques of sterilization of endodontic files. Glass bead sterilizer does not completely sterilize the files. The article also compares SteriFast and autoclave in other aspects such as its design, basic principle, advantages, and disadvantages. The article also describes features and design of SteriFast, used for all kind of small dental instruments. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5767831/ /pubmed/29386784 http://dx.doi.org/10.4103/JCD.JCD_253_16 Text en Copyright: © 2017 Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Research Article Sheth, Nomal Chintan Rathod, Yogesh V. Shenoi, Pratima R. Shori, Deepa D. Khode, Rajiv T. Khadse, Amruta P. Evaluation of new technique of sterilization using biological indicator |
title | Evaluation of new technique of sterilization using biological indicator |
title_full | Evaluation of new technique of sterilization using biological indicator |
title_fullStr | Evaluation of new technique of sterilization using biological indicator |
title_full_unstemmed | Evaluation of new technique of sterilization using biological indicator |
title_short | Evaluation of new technique of sterilization using biological indicator |
title_sort | evaluation of new technique of sterilization using biological indicator |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5767831/ https://www.ncbi.nlm.nih.gov/pubmed/29386784 http://dx.doi.org/10.4103/JCD.JCD_253_16 |
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