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Pixelated spatial gene expression analysis from tissue
Here, we present a technique that performs on-chip picoliter real-time reverse transcriptase loop mediated isothermal amplification (RT-LAMP) reactions on a histological tissue section without any analyte purification while preserving the native spatial location of the nucleic acid molecules. We dem...
Autores principales: | Ganguli, A., Ornob, A., Spegazzini, N., Liu, Y., Damhorst, G., Ghonge, T., Thornton, B., Konopka, C. J., Dobrucki, W., Clare, S. E., Bhargava, R., Smith, A. M., Kosari, F., Bashir, R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5768672/ https://www.ncbi.nlm.nih.gov/pubmed/29335461 http://dx.doi.org/10.1038/s41467-017-02623-9 |
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