Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status

A label-free microscopy method for assessing the differentiation status of stem cells is presented with potential application for characterization of therapeutic stem cell populations. The microscopy system is capable of characterizing live cells based on the use of evanescent wave microscopy and qu...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Jing, Moradi, Emilia, Somekh, Michael G., Mather, Melissa L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5768761/
https://www.ncbi.nlm.nih.gov/pubmed/29335508
http://dx.doi.org/10.1038/s41598-017-18714-y
_version_ 1783292767123800064
author Zhang, Jing
Moradi, Emilia
Somekh, Michael G.
Mather, Melissa L.
author_facet Zhang, Jing
Moradi, Emilia
Somekh, Michael G.
Mather, Melissa L.
author_sort Zhang, Jing
collection PubMed
description A label-free microscopy method for assessing the differentiation status of stem cells is presented with potential application for characterization of therapeutic stem cell populations. The microscopy system is capable of characterizing live cells based on the use of evanescent wave microscopy and quantitative phase contrast (QPC) microscopy. The capability of the microscopy system is demonstrated by studying the differentiation of live immortalised neonatal mouse neural stem cells over a 15 day time course. Metrics extracted from microscope images are assessed and images compared with results from endpoint immuno-staining studies to illustrate the system’s performance. Results demonstrate the potential of the microscopy system as a valuable tool for cell biologists to readily identify the differentiation status of unlabelled live cells.
format Online
Article
Text
id pubmed-5768761
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57687612018-01-25 Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status Zhang, Jing Moradi, Emilia Somekh, Michael G. Mather, Melissa L. Sci Rep Article A label-free microscopy method for assessing the differentiation status of stem cells is presented with potential application for characterization of therapeutic stem cell populations. The microscopy system is capable of characterizing live cells based on the use of evanescent wave microscopy and quantitative phase contrast (QPC) microscopy. The capability of the microscopy system is demonstrated by studying the differentiation of live immortalised neonatal mouse neural stem cells over a 15 day time course. Metrics extracted from microscope images are assessed and images compared with results from endpoint immuno-staining studies to illustrate the system’s performance. Results demonstrate the potential of the microscopy system as a valuable tool for cell biologists to readily identify the differentiation status of unlabelled live cells. Nature Publishing Group UK 2018-01-15 /pmc/articles/PMC5768761/ /pubmed/29335508 http://dx.doi.org/10.1038/s41598-017-18714-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Jing
Moradi, Emilia
Somekh, Michael G.
Mather, Melissa L.
Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status
title Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status
title_full Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status
title_fullStr Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status
title_full_unstemmed Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status
title_short Label-Free, High Resolution, Multi-Modal Light Microscopy for Discrimination of Live Stem Cell Differentiation Status
title_sort label-free, high resolution, multi-modal light microscopy for discrimination of live stem cell differentiation status
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5768761/
https://www.ncbi.nlm.nih.gov/pubmed/29335508
http://dx.doi.org/10.1038/s41598-017-18714-y
work_keys_str_mv AT zhangjing labelfreehighresolutionmultimodallightmicroscopyfordiscriminationoflivestemcelldifferentiationstatus
AT moradiemilia labelfreehighresolutionmultimodallightmicroscopyfordiscriminationoflivestemcelldifferentiationstatus
AT somekhmichaelg labelfreehighresolutionmultimodallightmicroscopyfordiscriminationoflivestemcelldifferentiationstatus
AT mathermelissal labelfreehighresolutionmultimodallightmicroscopyfordiscriminationoflivestemcelldifferentiationstatus

Ejemplares similares