Cargando…
Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of the Ascidian Halocynthia roretzi
Ascidian embryos have become an important model for embryological studies, offering a simple example for mechanisms of cytoplasmic components segregation. It is a well-known example that the asymmetric segregation of mitochondria into muscle lineage cells occurs during ascidian embryogenesis. Howeve...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Developmental Biology
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769141/ https://www.ncbi.nlm.nih.gov/pubmed/29354792 http://dx.doi.org/10.12717/DR.2017.21.4.467 |
_version_ | 1783292844985810944 |
---|---|
author | Baek, Yong Han Lee, Wang Jong Kim, Gil Jung |
author_facet | Baek, Yong Han Lee, Wang Jong Kim, Gil Jung |
author_sort | Baek, Yong Han |
collection | PubMed |
description | Ascidian embryos have become an important model for embryological studies, offering a simple example for mechanisms of cytoplasmic components segregation. It is a well-known example that the asymmetric segregation of mitochondria into muscle lineage cells occurs during ascidian embryogenesis. However, it is still unclear which signaling pathway is involved in this process. To obtain molecular markers for studying mechanisms involved in the asymmetric distribution of mitochondria, we have produced monoclonal antibodies, Mito-1, Mito-2 and Mito-3, that specifically recognize mitochondriarich cytoplasm in cells of the ascidian Halocynthia roretzi embryos. These antibodies stained cytoplasm like reticular structure in epidermis cells, except for nuclei, at the early tailbud stage. Similar pattern was observed in vital staining of mitochondria with DiOC(2), a fluorescent probe of mitochondria. Immunostaining with these antibodies showed that mitochondria are evenly distributed in the animal hemisphere blastomeres at cleavage stages, whereas not in the vegetal hemisphere blastomeres. Mitochondria were transferred to the presumptive muscle and nerve cord lineage cells of the marginal zone in the vegetal hemisphere more than to the presumptive mesenchyme, notochord and endoderm lineage of the central zone. Therefore, it is suggested that these antibodies will be useful markers for studying mechanisms involved in the polarized distribution of mitochondria during ascidian embryogenesis. |
format | Online Article Text |
id | pubmed-5769141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-57691412018-01-19 Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of the Ascidian Halocynthia roretzi Baek, Yong Han Lee, Wang Jong Kim, Gil Jung Dev Reprod Short Communication Ascidian embryos have become an important model for embryological studies, offering a simple example for mechanisms of cytoplasmic components segregation. It is a well-known example that the asymmetric segregation of mitochondria into muscle lineage cells occurs during ascidian embryogenesis. However, it is still unclear which signaling pathway is involved in this process. To obtain molecular markers for studying mechanisms involved in the asymmetric distribution of mitochondria, we have produced monoclonal antibodies, Mito-1, Mito-2 and Mito-3, that specifically recognize mitochondriarich cytoplasm in cells of the ascidian Halocynthia roretzi embryos. These antibodies stained cytoplasm like reticular structure in epidermis cells, except for nuclei, at the early tailbud stage. Similar pattern was observed in vital staining of mitochondria with DiOC(2), a fluorescent probe of mitochondria. Immunostaining with these antibodies showed that mitochondria are evenly distributed in the animal hemisphere blastomeres at cleavage stages, whereas not in the vegetal hemisphere blastomeres. Mitochondria were transferred to the presumptive muscle and nerve cord lineage cells of the marginal zone in the vegetal hemisphere more than to the presumptive mesenchyme, notochord and endoderm lineage of the central zone. Therefore, it is suggested that these antibodies will be useful markers for studying mechanisms involved in the polarized distribution of mitochondria during ascidian embryogenesis. The Korean Society of Developmental Biology 2017-12 2017-12-31 /pmc/articles/PMC5769141/ /pubmed/29354792 http://dx.doi.org/10.12717/DR.2017.21.4.467 Text en ⓒ Copyright 2017 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Baek, Yong Han Lee, Wang Jong Kim, Gil Jung Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of the Ascidian Halocynthia roretzi |
title | Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of
the Ascidian Halocynthia roretzi |
title_full | Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of
the Ascidian Halocynthia roretzi |
title_fullStr | Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of
the Ascidian Halocynthia roretzi |
title_full_unstemmed | Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of
the Ascidian Halocynthia roretzi |
title_short | Mitochondria-Specific Monoclonal Antibodies in Eggs and Embryos of
the Ascidian Halocynthia roretzi |
title_sort | mitochondria-specific monoclonal antibodies in eggs and embryos of
the ascidian halocynthia roretzi |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769141/ https://www.ncbi.nlm.nih.gov/pubmed/29354792 http://dx.doi.org/10.12717/DR.2017.21.4.467 |
work_keys_str_mv | AT baekyonghan mitochondriaspecificmonoclonalantibodiesineggsandembryosoftheascidianhalocynthiaroretzi AT leewangjong mitochondriaspecificmonoclonalantibodiesineggsandembryosoftheascidianhalocynthiaroretzi AT kimgiljung mitochondriaspecificmonoclonalantibodiesineggsandembryosoftheascidianhalocynthiaroretzi |