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Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology

Vibrio qinghaiensis sp.-Q67 (Vqin-Q67) is a freshwater luminescent bacterium that continuously emits blue-green light (485 nm). The bacterium has been widely used for detecting toxic contaminants. Here, we report the complete genome sequence of Vqin-Q67, obtained using third-generation PacBio sequen...

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Autores principales: Gong, Liang, Wu, Yu, Jian, Qijie, Yin, Chunxiao, Li, Taotao, Gupta, Vijai Kumar, Duan, Xuewu, Jiang, Yueming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769541/
https://www.ncbi.nlm.nih.gov/pubmed/29337313
http://dx.doi.org/10.1038/sdata.2017.205
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author Gong, Liang
Wu, Yu
Jian, Qijie
Yin, Chunxiao
Li, Taotao
Gupta, Vijai Kumar
Duan, Xuewu
Jiang, Yueming
author_facet Gong, Liang
Wu, Yu
Jian, Qijie
Yin, Chunxiao
Li, Taotao
Gupta, Vijai Kumar
Duan, Xuewu
Jiang, Yueming
author_sort Gong, Liang
collection PubMed
description Vibrio qinghaiensis sp.-Q67 (Vqin-Q67) is a freshwater luminescent bacterium that continuously emits blue-green light (485 nm). The bacterium has been widely used for detecting toxic contaminants. Here, we report the complete genome sequence of Vqin-Q67, obtained using third-generation PacBio sequencing technology. Continuous long reads were attained from three PacBio sequencing runs and reads >500 bp with a quality value of >0.75 were merged together into a single dataset. This resultant highly-contiguous de novo assembly has no genome gaps, and comprises two chromosomes with substantial genetic information, including protein-coding genes, non-coding RNA, transposon and gene islands. Our dataset can be useful as a comparative genome for evolution and speciation studies, as well as for the analysis of protein-coding gene families, the pathogenicity of different Vibrio species in fish, the evolution of non-coding RNA and transposon, and the regulation of gene expression in relation to the bioluminescence of Vqin-Q67.
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spelling pubmed-57695412018-01-29 Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology Gong, Liang Wu, Yu Jian, Qijie Yin, Chunxiao Li, Taotao Gupta, Vijai Kumar Duan, Xuewu Jiang, Yueming Sci Data Data Descriptor Vibrio qinghaiensis sp.-Q67 (Vqin-Q67) is a freshwater luminescent bacterium that continuously emits blue-green light (485 nm). The bacterium has been widely used for detecting toxic contaminants. Here, we report the complete genome sequence of Vqin-Q67, obtained using third-generation PacBio sequencing technology. Continuous long reads were attained from three PacBio sequencing runs and reads >500 bp with a quality value of >0.75 were merged together into a single dataset. This resultant highly-contiguous de novo assembly has no genome gaps, and comprises two chromosomes with substantial genetic information, including protein-coding genes, non-coding RNA, transposon and gene islands. Our dataset can be useful as a comparative genome for evolution and speciation studies, as well as for the analysis of protein-coding gene families, the pathogenicity of different Vibrio species in fish, the evolution of non-coding RNA and transposon, and the regulation of gene expression in relation to the bioluminescence of Vqin-Q67. Nature Publishing Group 2018-01-16 /pmc/articles/PMC5769541/ /pubmed/29337313 http://dx.doi.org/10.1038/sdata.2017.205 Text en Copyright © 2018, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.
spellingShingle Data Descriptor
Gong, Liang
Wu, Yu
Jian, Qijie
Yin, Chunxiao
Li, Taotao
Gupta, Vijai Kumar
Duan, Xuewu
Jiang, Yueming
Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology
title Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology
title_full Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology
title_fullStr Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology
title_full_unstemmed Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology
title_short Complete genome sequencing of the luminescent bacterium, Vibrio qinghaiensis sp. Q67 using PacBio technology
title_sort complete genome sequencing of the luminescent bacterium, vibrio qinghaiensis sp. q67 using pacbio technology
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769541/
https://www.ncbi.nlm.nih.gov/pubmed/29337313
http://dx.doi.org/10.1038/sdata.2017.205
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