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Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples

AIM: The epithelial layer within the colon represents a physical barrier between the luminal contents and its underlying mucosa. It plays a pivotal role in mucosal homeostasis, and both tolerance and anti-pathogenic immune responses. Identifying signals of inflammation initiation and responses to st...

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Detalles Bibliográficos
Autores principales: Balfe, Aine, Lennon, Grainne, Lavelle, Aonghus, Docherty, Neil G, Coffey, J Calvin, Sheahan, Kieran, Winter, Desmond C, O’Connell, P Ronan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769583/
https://www.ncbi.nlm.nih.gov/pubmed/29391821
http://dx.doi.org/10.2147/CEG.S145224