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Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart
Myocardial contractility and blood flow provide essential mechanical cues for the morphogenesis of the heart. In general, endothelial cells change their migratory behavior in response to shear stress patterns, according to flow directionality. Here, we assessed the impact of shear stress patterns an...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769631/ https://www.ncbi.nlm.nih.gov/pubmed/29183943 http://dx.doi.org/10.1242/dev.152124 |
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author | Boselli, Francesco Steed, Emily Freund, Jonathan B. Vermot, Julien |
author_facet | Boselli, Francesco Steed, Emily Freund, Jonathan B. Vermot, Julien |
author_sort | Boselli, Francesco |
collection | PubMed |
description | Myocardial contractility and blood flow provide essential mechanical cues for the morphogenesis of the heart. In general, endothelial cells change their migratory behavior in response to shear stress patterns, according to flow directionality. Here, we assessed the impact of shear stress patterns and flow directionality on the behavior of endocardial cells, the specialized endothelial cells of the heart. At the early stages of zebrafish heart valve formation, we show that endocardial cells are converging to the valve-forming area and that this behavior depends upon mechanical forces. Quantitative live imaging and mathematical modeling allow us to correlate this tissue convergence with the underlying flow forces. We predict that tissue convergence is associated with the direction of the mean wall shear stress and of the gradient of harmonic phase-averaged shear stresses, which surprisingly do not match the overall direction of the flow. This contrasts with the usual role of flow directionality in vascular development and suggests that the full spatial and temporal complexity of the wall shear stress should be taken into account when studying endothelial cell responses to flow in vivo. |
format | Online Article Text |
id | pubmed-5769631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57696312018-01-25 Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart Boselli, Francesco Steed, Emily Freund, Jonathan B. Vermot, Julien Development Research Report Myocardial contractility and blood flow provide essential mechanical cues for the morphogenesis of the heart. In general, endothelial cells change their migratory behavior in response to shear stress patterns, according to flow directionality. Here, we assessed the impact of shear stress patterns and flow directionality on the behavior of endocardial cells, the specialized endothelial cells of the heart. At the early stages of zebrafish heart valve formation, we show that endocardial cells are converging to the valve-forming area and that this behavior depends upon mechanical forces. Quantitative live imaging and mathematical modeling allow us to correlate this tissue convergence with the underlying flow forces. We predict that tissue convergence is associated with the direction of the mean wall shear stress and of the gradient of harmonic phase-averaged shear stresses, which surprisingly do not match the overall direction of the flow. This contrasts with the usual role of flow directionality in vascular development and suggests that the full spatial and temporal complexity of the wall shear stress should be taken into account when studying endothelial cell responses to flow in vivo. The Company of Biologists Ltd 2017-12-01 /pmc/articles/PMC5769631/ /pubmed/29183943 http://dx.doi.org/10.1242/dev.152124 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Report Boselli, Francesco Steed, Emily Freund, Jonathan B. Vermot, Julien Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
title | Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
title_full | Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
title_fullStr | Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
title_full_unstemmed | Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
title_short | Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
title_sort | anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart |
topic | Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5769631/ https://www.ncbi.nlm.nih.gov/pubmed/29183943 http://dx.doi.org/10.1242/dev.152124 |
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