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Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein

Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine effi...

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Autores principales: Kim, Ah-Ra, Lee, Dong-Hun, Lee, Su-Hwa, Rubino, Ilaria, Choi, Hyo-Jick, Quan, Fu-Shi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770062/
https://www.ncbi.nlm.nih.gov/pubmed/29338045
http://dx.doi.org/10.1371/journal.pone.0191277
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author Kim, Ah-Ra
Lee, Dong-Hun
Lee, Su-Hwa
Rubino, Ilaria
Choi, Hyo-Jick
Quan, Fu-Shi
author_facet Kim, Ah-Ra
Lee, Dong-Hun
Lee, Su-Hwa
Rubino, Ilaria
Choi, Hyo-Jick
Quan, Fu-Shi
author_sort Kim, Ah-Ra
collection PubMed
description Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine efficacy in a mouse model. For VLP vaccines, tandem gene (1–780 bp) for V1 VLPs and tandem repeat gene (repeated 450–780 bp) for V5 VLPs were constructed in pFastBac(TM) vectors, respectively. Influenza matrix protein 1 (M1) was used as a core protein in the VLPs. Notably, upon challenge infection, significantly lower virus loads were measured in the lung of mice immunized with V1 or V5 VLPs compared to those of naïve mice and formalin-inactivated RSV immunized control mice. In particular, V5 VLPs immunization showed significantly lower virus titers than V1 VLPs immunization. Furthermore, V5 VLPs immunization elicited increased memory B cells responses in the spleen. These results indicated that V5 VLP vaccine containing tandem repeat gene protein provided better protection than V1 VLPs with significantly decreased inflammation in the lungs. Thus, V5 VLPs could be a potential vaccine candidate against RSV.
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spelling pubmed-57700622018-01-23 Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein Kim, Ah-Ra Lee, Dong-Hun Lee, Su-Hwa Rubino, Ilaria Choi, Hyo-Jick Quan, Fu-Shi PLoS One Research Article Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine efficacy in a mouse model. For VLP vaccines, tandem gene (1–780 bp) for V1 VLPs and tandem repeat gene (repeated 450–780 bp) for V5 VLPs were constructed in pFastBac(TM) vectors, respectively. Influenza matrix protein 1 (M1) was used as a core protein in the VLPs. Notably, upon challenge infection, significantly lower virus loads were measured in the lung of mice immunized with V1 or V5 VLPs compared to those of naïve mice and formalin-inactivated RSV immunized control mice. In particular, V5 VLPs immunization showed significantly lower virus titers than V1 VLPs immunization. Furthermore, V5 VLPs immunization elicited increased memory B cells responses in the spleen. These results indicated that V5 VLP vaccine containing tandem repeat gene protein provided better protection than V1 VLPs with significantly decreased inflammation in the lungs. Thus, V5 VLPs could be a potential vaccine candidate against RSV. Public Library of Science 2018-01-16 /pmc/articles/PMC5770062/ /pubmed/29338045 http://dx.doi.org/10.1371/journal.pone.0191277 Text en © 2018 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kim, Ah-Ra
Lee, Dong-Hun
Lee, Su-Hwa
Rubino, Ilaria
Choi, Hyo-Jick
Quan, Fu-Shi
Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein
title Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein
title_full Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein
title_fullStr Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein
title_full_unstemmed Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein
title_short Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein
title_sort protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus g protein
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770062/
https://www.ncbi.nlm.nih.gov/pubmed/29338045
http://dx.doi.org/10.1371/journal.pone.0191277
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