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Structure and function of urea amidolyase
Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770610/ https://www.ncbi.nlm.nih.gov/pubmed/29263142 http://dx.doi.org/10.1042/BSR20171617 |
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author | Zhao, Jing Zhu, Li Fan, Chen Wu, Yi Xiang, Song |
author_facet | Zhao, Jing Zhu, Li Fan, Chen Wu, Yi Xiang, Song |
author_sort | Zhao, Jing |
collection | PubMed |
description | Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) and allophanate hydrolase (AH) domains, which catalyze sequential reactions. In some organisms UC and AH are encoded by separated genes. We present here structure of the Kluyveromyces lactis UA (KlUA). The structure revealed that KlUA forms a compact homo-dimer with a molecular weight of 400 kDa. Structure inspired biochemical experiments revealed the mechanism of its reaction intermediate translocation, and that the KlUA holo-enzyme formation is essential for its optimal activity. Interestingly, previous studies and ours suggest that UC and AH encoded by separated genes probably do not form a KlUA-like complex, consequently they might not catalyze the urea to ammonium conversion as efficiently. |
format | Online Article Text |
id | pubmed-5770610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57706102018-01-29 Structure and function of urea amidolyase Zhao, Jing Zhu, Li Fan, Chen Wu, Yi Xiang, Song Biosci Rep Research Articles Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) and allophanate hydrolase (AH) domains, which catalyze sequential reactions. In some organisms UC and AH are encoded by separated genes. We present here structure of the Kluyveromyces lactis UA (KlUA). The structure revealed that KlUA forms a compact homo-dimer with a molecular weight of 400 kDa. Structure inspired biochemical experiments revealed the mechanism of its reaction intermediate translocation, and that the KlUA holo-enzyme formation is essential for its optimal activity. Interestingly, previous studies and ours suggest that UC and AH encoded by separated genes probably do not form a KlUA-like complex, consequently they might not catalyze the urea to ammonium conversion as efficiently. Portland Press Ltd. 2018-01-17 /pmc/articles/PMC5770610/ /pubmed/29263142 http://dx.doi.org/10.1042/BSR20171617 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Zhao, Jing Zhu, Li Fan, Chen Wu, Yi Xiang, Song Structure and function of urea amidolyase |
title | Structure and function of urea amidolyase |
title_full | Structure and function of urea amidolyase |
title_fullStr | Structure and function of urea amidolyase |
title_full_unstemmed | Structure and function of urea amidolyase |
title_short | Structure and function of urea amidolyase |
title_sort | structure and function of urea amidolyase |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770610/ https://www.ncbi.nlm.nih.gov/pubmed/29263142 http://dx.doi.org/10.1042/BSR20171617 |
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