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Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition

Atmospheric nitrogen (N) deposition is changing in both load quantity and chemical composition. The load effects have been studied extensively, whereas the composition effects remain poorly understood. We conducted a microcosm experiment to study how N chemistry affected the soil microbial community...

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Detalles Bibliográficos
Autores principales: Wei, Hui, Chen, Xiaomei, He, Jinhong, Zhang, Jiaen, Shen, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770617/
https://www.ncbi.nlm.nih.gov/pubmed/29375484
http://dx.doi.org/10.3389/fmicb.2017.02382
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author Wei, Hui
Chen, Xiaomei
He, Jinhong
Zhang, Jiaen
Shen, Weijun
author_facet Wei, Hui
Chen, Xiaomei
He, Jinhong
Zhang, Jiaen
Shen, Weijun
author_sort Wei, Hui
collection PubMed
description Atmospheric nitrogen (N) deposition is changing in both load quantity and chemical composition. The load effects have been studied extensively, whereas the composition effects remain poorly understood. We conducted a microcosm experiment to study how N chemistry affected the soil microbial community composition characterized by phospholipid fatty acids (PLFAs) and activity indicated by microbial CO(2) release. Surface and subsurface soils collected from an old-growth subtropical forest were supplemented with three N-containing materials (ammonium, nitrate, and urea) at the current regional deposition load (50 kg ha(-1) yr(-1)) and incubated at three temperatures (10, 20, and 30°C) to detect the interactive effects of N deposition and temperature. The results showed that the additions of N, regardless of form, did not alter the microbial PLFAs at any of the three temperatures. However, the addition of urea significantly stimulated soil CO(2) release in the early incubation stage. Compared with the control, N addition consistently reduced the temperature dependency of microbial respiration, implying that N deposition could potentially weaken the positive feedback of the warming-stimulated soil CO(2) release to the atmosphere. The consistent N effects for the surface and subsurface soils suggest that the effects of N on soil microbial communities may be independent of soil chemical contents and stoichiometry.
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spelling pubmed-57706172018-01-26 Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition Wei, Hui Chen, Xiaomei He, Jinhong Zhang, Jiaen Shen, Weijun Front Microbiol Microbiology Atmospheric nitrogen (N) deposition is changing in both load quantity and chemical composition. The load effects have been studied extensively, whereas the composition effects remain poorly understood. We conducted a microcosm experiment to study how N chemistry affected the soil microbial community composition characterized by phospholipid fatty acids (PLFAs) and activity indicated by microbial CO(2) release. Surface and subsurface soils collected from an old-growth subtropical forest were supplemented with three N-containing materials (ammonium, nitrate, and urea) at the current regional deposition load (50 kg ha(-1) yr(-1)) and incubated at three temperatures (10, 20, and 30°C) to detect the interactive effects of N deposition and temperature. The results showed that the additions of N, regardless of form, did not alter the microbial PLFAs at any of the three temperatures. However, the addition of urea significantly stimulated soil CO(2) release in the early incubation stage. Compared with the control, N addition consistently reduced the temperature dependency of microbial respiration, implying that N deposition could potentially weaken the positive feedback of the warming-stimulated soil CO(2) release to the atmosphere. The consistent N effects for the surface and subsurface soils suggest that the effects of N on soil microbial communities may be independent of soil chemical contents and stoichiometry. Frontiers Media S.A. 2017-12-01 /pmc/articles/PMC5770617/ /pubmed/29375484 http://dx.doi.org/10.3389/fmicb.2017.02382 Text en Copyright © 2017 Wei, Chen, He, Zhang and Shen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wei, Hui
Chen, Xiaomei
He, Jinhong
Zhang, Jiaen
Shen, Weijun
Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition
title Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition
title_full Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition
title_fullStr Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition
title_full_unstemmed Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition
title_short Exogenous Nitrogen Addition Reduced the Temperature Sensitivity of Microbial Respiration without Altering the Microbial Community Composition
title_sort exogenous nitrogen addition reduced the temperature sensitivity of microbial respiration without altering the microbial community composition
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770617/
https://www.ncbi.nlm.nih.gov/pubmed/29375484
http://dx.doi.org/10.3389/fmicb.2017.02382
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