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Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes

AIM: The main purpose of the present study was to test the β-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out b...

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Detalles Bibliográficos
Autores principales: Zarrin, Majid, Rashidnia, Zeinab, Faramarzi, Sama, Harooni, Lida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771284/
https://www.ncbi.nlm.nih.gov/pubmed/29362608
http://dx.doi.org/10.3889/oamjms.2017.203
Descripción
Sumario:AIM: The main purpose of the present study was to test the β-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on βtub and rodA partial gene sequences. RESULTS: A 198 bp DNA fragment was obtained using βtub gene. PCR amplification of the rodA gene resulted in a 313 bp band. The βtub and rodA genes PCR products exhibited a 100% homology with the associated sequences in the GenBank. CONCLUSION: In the present study, we used a PCR approach that was able to discriminate A. fumigatus from other related species within the section Fumigati.