Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes

AIM: The main purpose of the present study was to test the β-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out b...

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Autores principales: Zarrin, Majid, Rashidnia, Zeinab, Faramarzi, Sama, Harooni, Lida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Republic of Macedonia 2017
Materias:
Basic Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771284/
https://www.ncbi.nlm.nih.gov/pubmed/29362608
http://dx.doi.org/10.3889/oamjms.2017.203
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author Zarrin, Majid
Rashidnia, Zeinab
Faramarzi, Sama
Harooni, Lida
author_facet Zarrin, Majid
Rashidnia, Zeinab
Faramarzi, Sama
Harooni, Lida
author_sort Zarrin, Majid
collection PubMed
description AIM: The main purpose of the present study was to test the β-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on βtub and rodA partial gene sequences. RESULTS: A 198 bp DNA fragment was obtained using βtub gene. PCR amplification of the rodA gene resulted in a 313 bp band. The βtub and rodA genes PCR products exhibited a 100% homology with the associated sequences in the GenBank. CONCLUSION: In the present study, we used a PCR approach that was able to discriminate A. fumigatus from other related species within the section Fumigati.
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spelling pubmed-57712842018-01-23 Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes Zarrin, Majid Rashidnia, Zeinab Faramarzi, Sama Harooni, Lida Open Access Maced J Med Sci Basic Science AIM: The main purpose of the present study was to test the β-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS: Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on βtub and rodA partial gene sequences. RESULTS: A 198 bp DNA fragment was obtained using βtub gene. PCR amplification of the rodA gene resulted in a 313 bp band. The βtub and rodA genes PCR products exhibited a 100% homology with the associated sequences in the GenBank. CONCLUSION: In the present study, we used a PCR approach that was able to discriminate A. fumigatus from other related species within the section Fumigati. Republic of Macedonia 2017-11-22 /pmc/articles/PMC5771284/ /pubmed/29362608 http://dx.doi.org/10.3889/oamjms.2017.203 Text en Copyright: © 2017 Majid Zarrin, Zeinab Rashidnia, Sama Faramarzi, Lida Harooni. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
spellingShingle Basic Science
Zarrin, Majid
Rashidnia, Zeinab
Faramarzi, Sama
Harooni, Lida
Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
title Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
title_full Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
title_fullStr Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
title_full_unstemmed Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
title_short Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
title_sort rapid identification of aspergillus fumigatus using βeta-tubulin and rodleta genes
topic Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771284/
https://www.ncbi.nlm.nih.gov/pubmed/29362608
http://dx.doi.org/10.3889/oamjms.2017.203
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