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Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer
Krt17 is a 48kDa protein member of keratin family. Previous literatures have demonstrated Krt17 may play a promotive role in the progression of various malignancies. However, the exact function of Krt17 in the carcinogenesis and the progression of gastric cancer (GC) remains unknown. In the present...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771342/ https://www.ncbi.nlm.nih.gov/pubmed/29344281 http://dx.doi.org/10.7150/jca.19838 |
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author | Hu, Hao Xu, Dan-hua Huang, Xiao-xu Zhu, Chun-chao Xu, Jia Zhang, Zi-zhen Zhao, Gang |
author_facet | Hu, Hao Xu, Dan-hua Huang, Xiao-xu Zhu, Chun-chao Xu, Jia Zhang, Zi-zhen Zhao, Gang |
author_sort | Hu, Hao |
collection | PubMed |
description | Krt17 is a 48kDa protein member of keratin family. Previous literatures have demonstrated Krt17 may play a promotive role in the progression of various malignancies. However, the exact function of Krt17 in the carcinogenesis and the progression of gastric cancer (GC) remains unknown. In the present study, the expression of Krt17 in 20 fresh GC and matched normal tissues were detected and Krt17 was found to be significantly increased in GC tissues compared to normal tissues. And then the immunochemistry was performed to investigate the Krt17 expression in 569 GC tissue specimens, we found that the expression of Krt17 was remarkably positively correlated with the tumor size (P < 0.01), depth of invasion (T) (P < 0.001), lymph node metastasis (N) (P < 0.001), tumor node metastasis (TNM) stage (P < 0.001) and vascular invasion (P < 0.05). High expression of Krt17 predicted a poor prognosis of GC patients. In addition, we showed silencing of Krt17 inhibited GC cell proliferation, migration and invasion, and induced cell apoptosis by altering Bcl2 family protein expression and cleaved caspase3 upregulation. Moreover, silencing of Krt17 led to cell cycle arrest at G1/S stage by decreasing cyclin E1 and cyclin D expression. In conclusion, our findings revealed Krt17 can be used as a novel predictive biomarker, thus providing a novel therapeutic target for GC patients. |
format | Online Article Text |
id | pubmed-5771342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-57713422018-01-17 Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer Hu, Hao Xu, Dan-hua Huang, Xiao-xu Zhu, Chun-chao Xu, Jia Zhang, Zi-zhen Zhao, Gang J Cancer Research Paper Krt17 is a 48kDa protein member of keratin family. Previous literatures have demonstrated Krt17 may play a promotive role in the progression of various malignancies. However, the exact function of Krt17 in the carcinogenesis and the progression of gastric cancer (GC) remains unknown. In the present study, the expression of Krt17 in 20 fresh GC and matched normal tissues were detected and Krt17 was found to be significantly increased in GC tissues compared to normal tissues. And then the immunochemistry was performed to investigate the Krt17 expression in 569 GC tissue specimens, we found that the expression of Krt17 was remarkably positively correlated with the tumor size (P < 0.01), depth of invasion (T) (P < 0.001), lymph node metastasis (N) (P < 0.001), tumor node metastasis (TNM) stage (P < 0.001) and vascular invasion (P < 0.05). High expression of Krt17 predicted a poor prognosis of GC patients. In addition, we showed silencing of Krt17 inhibited GC cell proliferation, migration and invasion, and induced cell apoptosis by altering Bcl2 family protein expression and cleaved caspase3 upregulation. Moreover, silencing of Krt17 led to cell cycle arrest at G1/S stage by decreasing cyclin E1 and cyclin D expression. In conclusion, our findings revealed Krt17 can be used as a novel predictive biomarker, thus providing a novel therapeutic target for GC patients. Ivyspring International Publisher 2018-01-01 /pmc/articles/PMC5771342/ /pubmed/29344281 http://dx.doi.org/10.7150/jca.19838 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Hu, Hao Xu, Dan-hua Huang, Xiao-xu Zhu, Chun-chao Xu, Jia Zhang, Zi-zhen Zhao, Gang Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer |
title | Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer |
title_full | Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer |
title_fullStr | Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer |
title_full_unstemmed | Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer |
title_short | Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer |
title_sort | keratin17 promotes tumor growth and is associated with poor prognosis in gastric cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771342/ https://www.ncbi.nlm.nih.gov/pubmed/29344281 http://dx.doi.org/10.7150/jca.19838 |
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