A Survey of Validation Strategies for CRISPR-Cas9 Editing

The T7 endonuclease 1 (T7E1) mismatch detection assay is a widely used method for evaluating the activity of site-specific nucleases, such as the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system. To determine the accuracy and sensitivity of this assay, we compared the e...

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Detalles Bibliográficos
Autores principales: Sentmanat, Monica F., Peters, Samuel T., Florian, Colin P., Connelly, Jon P., Pruett-Miller, Shondra M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772360/
https://www.ncbi.nlm.nih.gov/pubmed/29343825
http://dx.doi.org/10.1038/s41598-018-19441-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772360/
https://www.ncbi.nlm.nih.gov/pubmed/29343825
http://dx.doi.org/10.1038/s41598-018-19441-8

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