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Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma

CD14(+) monocytes contain precursors for macrophages and fibrocytes, known to be involved in regulating airway remodeling in human asthma and distinguishable by the PM-2K marker. We sought to identify circulating subsets of PM-2K(+) macrophage-like cells and evaluate their relationships to lung func...

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Detalles Bibliográficos
Autores principales: Hung, Chih-Hsing, Wang, Chin-Chou, Suen, Jau-Ling, Sheu, Chau-Chyun, Kuo, Chang-Hung, Liao, Wei-Ting, Yang, Yi-Hsin, Wu, Chao-Chien, Leung, Sum-Yee, Lai, Ruay-Sheng, Lin, Chi-Cheng, Wei, Yu-Feng, Lee, Chong-Yeh, Huang, Ming-Shyan, Huang, Shau-Ku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772494/
https://www.ncbi.nlm.nih.gov/pubmed/29343695
http://dx.doi.org/10.1038/s41598-017-19105-z
Descripción
Sumario:CD14(+) monocytes contain precursors for macrophages and fibrocytes, known to be involved in regulating airway remodeling in human asthma and distinguishable by the PM-2K marker. We sought to identify circulating subsets of PM-2K(+) macrophage-like cells and evaluate their relationships to lung function, severity and control status. Circulating PM-2K(+) macrophage-like cells and fibrocytes could be identified and distinguished between normal individuals (N = 152) and asthmatic subjects (N = 133) using multi-parametric flow cytometry. PM-2K(+) macrophage-like cells were found to be significantly lower in asthmatic subjects, particularly noted for the CD14(−)PM-2K(+) subset and PM-2K(+)CCR7(−)CD86(+) cells in subjects with poor lung function (FEV%/FVC% < 80%) as compared to those of normal subjects and asthmatics with normal lung function, whereas the frequency of fibrocytes was higher in asthmatics and the CCR7(−)CD86(+) subset distribution was significantly different in subjects with varying severity. Moreover, exogenous transforming growth factor beta 1 (TGF-β1) was found to inhibit the generation of PM-2K(+) macrophage(-)like cells, but promote the growth of fibrocytes, from CD14(+) monocytes(,) and monocyte-derived TGF-β1 was found to correlate with the lung function, severity and control status in asthmatic patients. Collectively, aberrant differentiation of monocytes into PM-2K(+) macrophage-like cell subsets and fibrocytes, together with increased monocyte-derived TGF-β1, characterized patients with severe asthma.