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Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique

Bovine tuberculosis is a zoonotic disease with largely unknown impact in Africa, with risk factors such as HIV and direct contact with animals or consumption of Mycobacterium bovis infected animal products. In order to understand and quantify this risk and design intervention strategies, good epidem...

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Autores principales: Machado, Adelina, Rito, Teresa, Ghebremichael, Solomon, Muhate, Nuelma, Maxhuza, Gabriel, Macuamule, Custodia, Moiane, Ivania, Macucule, Baltazar, Marranangumbe, Angelica Suzana, Baptista, Jorge, Manguele, Joaquim, Koivula, Tuija, Streicher, Elizabeth Maria, Warren, Robin Mark, Kallenius, Gunilla, van Helden, Paul, Correia-Neves, Margarida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772998/
https://www.ncbi.nlm.nih.gov/pubmed/29346413
http://dx.doi.org/10.1371/journal.pntd.0006147
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author Machado, Adelina
Rito, Teresa
Ghebremichael, Solomon
Muhate, Nuelma
Maxhuza, Gabriel
Macuamule, Custodia
Moiane, Ivania
Macucule, Baltazar
Marranangumbe, Angelica Suzana
Baptista, Jorge
Manguele, Joaquim
Koivula, Tuija
Streicher, Elizabeth Maria
Warren, Robin Mark
Kallenius, Gunilla
van Helden, Paul
Correia-Neves, Margarida
author_facet Machado, Adelina
Rito, Teresa
Ghebremichael, Solomon
Muhate, Nuelma
Maxhuza, Gabriel
Macuamule, Custodia
Moiane, Ivania
Macucule, Baltazar
Marranangumbe, Angelica Suzana
Baptista, Jorge
Manguele, Joaquim
Koivula, Tuija
Streicher, Elizabeth Maria
Warren, Robin Mark
Kallenius, Gunilla
van Helden, Paul
Correia-Neves, Margarida
author_sort Machado, Adelina
collection PubMed
description Bovine tuberculosis is a zoonotic disease with largely unknown impact in Africa, with risk factors such as HIV and direct contact with animals or consumption of Mycobacterium bovis infected animal products. In order to understand and quantify this risk and design intervention strategies, good epidemiological studies are needed. Such studies can include molecular typing of M. bovis isolates. The aim of this study was to apply these tools to provide novel information concerning the distribution of bovine tuberculosis in cattle in Mozambique and thereby provide relevant information to guide policy development and strategies to contain the disease in livestock, and reduce the risk associated with transmission to humans. A collection of 178 M. bovis isolates was obtained from cattle in Mozambique. Using spoligotyping and regions of difference analysis, we classified the isolates into clonal complexes, thus reporting the first characterisation of M. bovis strains in this region. Data from MIRU-VNTR typing was used to compare isolates from a number of African countries, revealing a deeply geographically structured diversity of M. bovis. Eastern Africa appears to show high diversity, suggesting deep evolution in that region. The diversity of M. bovis in Africa does not seem to be a function of recent importation of animals, but is probably maintained within each particular region by constant reinfection from reservoir animals. Understanding the transmission routes of M. bovis in Mozambique and elsewhere is essential in order to focus public health and veterinary resources to contain bovine tuberculosis.
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spelling pubmed-57729982018-01-26 Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique Machado, Adelina Rito, Teresa Ghebremichael, Solomon Muhate, Nuelma Maxhuza, Gabriel Macuamule, Custodia Moiane, Ivania Macucule, Baltazar Marranangumbe, Angelica Suzana Baptista, Jorge Manguele, Joaquim Koivula, Tuija Streicher, Elizabeth Maria Warren, Robin Mark Kallenius, Gunilla van Helden, Paul Correia-Neves, Margarida PLoS Negl Trop Dis Research Article Bovine tuberculosis is a zoonotic disease with largely unknown impact in Africa, with risk factors such as HIV and direct contact with animals or consumption of Mycobacterium bovis infected animal products. In order to understand and quantify this risk and design intervention strategies, good epidemiological studies are needed. Such studies can include molecular typing of M. bovis isolates. The aim of this study was to apply these tools to provide novel information concerning the distribution of bovine tuberculosis in cattle in Mozambique and thereby provide relevant information to guide policy development and strategies to contain the disease in livestock, and reduce the risk associated with transmission to humans. A collection of 178 M. bovis isolates was obtained from cattle in Mozambique. Using spoligotyping and regions of difference analysis, we classified the isolates into clonal complexes, thus reporting the first characterisation of M. bovis strains in this region. Data from MIRU-VNTR typing was used to compare isolates from a number of African countries, revealing a deeply geographically structured diversity of M. bovis. Eastern Africa appears to show high diversity, suggesting deep evolution in that region. The diversity of M. bovis in Africa does not seem to be a function of recent importation of animals, but is probably maintained within each particular region by constant reinfection from reservoir animals. Understanding the transmission routes of M. bovis in Mozambique and elsewhere is essential in order to focus public health and veterinary resources to contain bovine tuberculosis. Public Library of Science 2018-01-18 /pmc/articles/PMC5772998/ /pubmed/29346413 http://dx.doi.org/10.1371/journal.pntd.0006147 Text en © 2018 Machado et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Machado, Adelina
Rito, Teresa
Ghebremichael, Solomon
Muhate, Nuelma
Maxhuza, Gabriel
Macuamule, Custodia
Moiane, Ivania
Macucule, Baltazar
Marranangumbe, Angelica Suzana
Baptista, Jorge
Manguele, Joaquim
Koivula, Tuija
Streicher, Elizabeth Maria
Warren, Robin Mark
Kallenius, Gunilla
van Helden, Paul
Correia-Neves, Margarida
Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
title Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
title_full Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
title_fullStr Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
title_full_unstemmed Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
title_short Genetic diversity and potential routes of transmission of Mycobacterium bovis in Mozambique
title_sort genetic diversity and potential routes of transmission of mycobacterium bovis in mozambique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772998/
https://www.ncbi.nlm.nih.gov/pubmed/29346413
http://dx.doi.org/10.1371/journal.pntd.0006147
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