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Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

OBJECTIVE: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis. METHODS: Animals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilatera...

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Autores principales: Halmenschlager, Graziele, Rhoden, Ernani Luis, Motta, Gabriela Almeida, Sagrillo Fagundes, Lucas, Medeiros, Jorge Luiz, Meurer, Rosalva, Rhoden, Cláudia Ramos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Second Military Medical University 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773047/
https://www.ncbi.nlm.nih.gov/pubmed/29387554
http://dx.doi.org/10.1016/j.ajur.2017.02.001
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author Halmenschlager, Graziele
Rhoden, Ernani Luis
Motta, Gabriela Almeida
Sagrillo Fagundes, Lucas
Medeiros, Jorge Luiz
Meurer, Rosalva
Rhoden, Cláudia Ramos
author_facet Halmenschlager, Graziele
Rhoden, Ernani Luis
Motta, Gabriela Almeida
Sagrillo Fagundes, Lucas
Medeiros, Jorge Luiz
Meurer, Rosalva
Rhoden, Cláudia Ramos
author_sort Halmenschlager, Graziele
collection PubMed
description OBJECTIVE: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis. METHODS: Animals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec + T group (n = 8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia. Azan's trichrome staining was used to evaluate smooth muscle, Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen. Apoptosis was evaluated using TUNEL technique. RESULTS: T levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001). T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001, respectively). No difference was observed in the content of sinusoidal space (p = 0.207), elastic fibers (p = 0.849), collagen (p = 0.216) and in apoptosis (p = 0.095). CONCLUSION: Normal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers, collagen content and apoptotic index. Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure.
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spelling pubmed-57730472018-01-31 Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats Halmenschlager, Graziele Rhoden, Ernani Luis Motta, Gabriela Almeida Sagrillo Fagundes, Lucas Medeiros, Jorge Luiz Meurer, Rosalva Rhoden, Cláudia Ramos Asian J Urol Original Article OBJECTIVE: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis. METHODS: Animals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec + T group (n = 8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia. Azan's trichrome staining was used to evaluate smooth muscle, Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen. Apoptosis was evaluated using TUNEL technique. RESULTS: T levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001). T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001, respectively). No difference was observed in the content of sinusoidal space (p = 0.207), elastic fibers (p = 0.849), collagen (p = 0.216) and in apoptosis (p = 0.095). CONCLUSION: Normal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers, collagen content and apoptotic index. Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure. Second Military Medical University 2017-10 2017-02-16 /pmc/articles/PMC5773047/ /pubmed/29387554 http://dx.doi.org/10.1016/j.ajur.2017.02.001 Text en © 2017 Editorial Office of Asian Journal of Urology. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Halmenschlager, Graziele
Rhoden, Ernani Luis
Motta, Gabriela Almeida
Sagrillo Fagundes, Lucas
Medeiros, Jorge Luiz
Meurer, Rosalva
Rhoden, Cláudia Ramos
Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
title Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
title_full Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
title_fullStr Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
title_full_unstemmed Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
title_short Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
title_sort testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773047/
https://www.ncbi.nlm.nih.gov/pubmed/29387554
http://dx.doi.org/10.1016/j.ajur.2017.02.001
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