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Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats
OBJECTIVE: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis. METHODS: Animals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilatera...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Second Military Medical University
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773047/ https://www.ncbi.nlm.nih.gov/pubmed/29387554 http://dx.doi.org/10.1016/j.ajur.2017.02.001 |
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author | Halmenschlager, Graziele Rhoden, Ernani Luis Motta, Gabriela Almeida Sagrillo Fagundes, Lucas Medeiros, Jorge Luiz Meurer, Rosalva Rhoden, Cláudia Ramos |
author_facet | Halmenschlager, Graziele Rhoden, Ernani Luis Motta, Gabriela Almeida Sagrillo Fagundes, Lucas Medeiros, Jorge Luiz Meurer, Rosalva Rhoden, Cláudia Ramos |
author_sort | Halmenschlager, Graziele |
collection | PubMed |
description | OBJECTIVE: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis. METHODS: Animals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec + T group (n = 8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia. Azan's trichrome staining was used to evaluate smooth muscle, Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen. Apoptosis was evaluated using TUNEL technique. RESULTS: T levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001). T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001, respectively). No difference was observed in the content of sinusoidal space (p = 0.207), elastic fibers (p = 0.849), collagen (p = 0.216) and in apoptosis (p = 0.095). CONCLUSION: Normal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers, collagen content and apoptotic index. Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure. |
format | Online Article Text |
id | pubmed-5773047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Second Military Medical University |
record_format | MEDLINE/PubMed |
spelling | pubmed-57730472018-01-31 Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats Halmenschlager, Graziele Rhoden, Ernani Luis Motta, Gabriela Almeida Sagrillo Fagundes, Lucas Medeiros, Jorge Luiz Meurer, Rosalva Rhoden, Cláudia Ramos Asian J Urol Original Article OBJECTIVE: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis. METHODS: Animals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec + T group (n = 8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia. Azan's trichrome staining was used to evaluate smooth muscle, Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen. Apoptosis was evaluated using TUNEL technique. RESULTS: T levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001). T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001, respectively). No difference was observed in the content of sinusoidal space (p = 0.207), elastic fibers (p = 0.849), collagen (p = 0.216) and in apoptosis (p = 0.095). CONCLUSION: Normal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers, collagen content and apoptotic index. Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure. Second Military Medical University 2017-10 2017-02-16 /pmc/articles/PMC5773047/ /pubmed/29387554 http://dx.doi.org/10.1016/j.ajur.2017.02.001 Text en © 2017 Editorial Office of Asian Journal of Urology. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Halmenschlager, Graziele Rhoden, Ernani Luis Motta, Gabriela Almeida Sagrillo Fagundes, Lucas Medeiros, Jorge Luiz Meurer, Rosalva Rhoden, Cláudia Ramos Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
title | Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
title_full | Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
title_fullStr | Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
title_full_unstemmed | Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
title_short | Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
title_sort | testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773047/ https://www.ncbi.nlm.nih.gov/pubmed/29387554 http://dx.doi.org/10.1016/j.ajur.2017.02.001 |
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