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RNase I(f) -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops
BACKGROUND: RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such as resistance against insects, fungi, viruses, and metabolism manipulation. To...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773123/ https://www.ncbi.nlm.nih.gov/pubmed/29343265 http://dx.doi.org/10.1186/s12896-018-0413-6 |