Cargando…

Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

BACKGROUND: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Molano, Eddy Patricia Lopez, Cabrera, Odalys García, Jose, Juliana, do Nascimento, Leandro Costa, Carazzolle, Marcelo Falsarella, Teixeira, Paulo José Pereira Lima, Alvarez, Javier Correa, Tiburcio, Ricardo Augusto, Tokimatu Filho, Paulo Massanari, de Lima, Gustavo Machado Alvares, Guido, Rafael Victório Carvalho, Corrêa, Thamy Lívia Ribeiro, Leme, Adriana Franco Paes, Mieczkowski, Piotr, Pereira, Gonçalo Amarante Guimarães
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773145/
https://www.ncbi.nlm.nih.gov/pubmed/29343217
http://dx.doi.org/10.1186/s12864-018-4440-4
_version_ 1783293510790676480
author Molano, Eddy Patricia Lopez
Cabrera, Odalys García
Jose, Juliana
do Nascimento, Leandro Costa
Carazzolle, Marcelo Falsarella
Teixeira, Paulo José Pereira Lima
Alvarez, Javier Correa
Tiburcio, Ricardo Augusto
Tokimatu Filho, Paulo Massanari
de Lima, Gustavo Machado Alvares
Guido, Rafael Victório Carvalho
Corrêa, Thamy Lívia Ribeiro
Leme, Adriana Franco Paes
Mieczkowski, Piotr
Pereira, Gonçalo Amarante Guimarães
author_facet Molano, Eddy Patricia Lopez
Cabrera, Odalys García
Jose, Juliana
do Nascimento, Leandro Costa
Carazzolle, Marcelo Falsarella
Teixeira, Paulo José Pereira Lima
Alvarez, Javier Correa
Tiburcio, Ricardo Augusto
Tokimatu Filho, Paulo Massanari
de Lima, Gustavo Machado Alvares
Guido, Rafael Victório Carvalho
Corrêa, Thamy Lívia Ribeiro
Leme, Adriana Franco Paes
Mieczkowski, Piotr
Pereira, Gonçalo Amarante Guimarães
author_sort Molano, Eddy Patricia Lopez
collection PubMed
description BACKGROUND: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. RESULTS: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC–MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. CONCLUSION: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4440-4) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5773145
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-57731452018-01-26 Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC) Molano, Eddy Patricia Lopez Cabrera, Odalys García Jose, Juliana do Nascimento, Leandro Costa Carazzolle, Marcelo Falsarella Teixeira, Paulo José Pereira Lima Alvarez, Javier Correa Tiburcio, Ricardo Augusto Tokimatu Filho, Paulo Massanari de Lima, Gustavo Machado Alvares Guido, Rafael Victório Carvalho Corrêa, Thamy Lívia Ribeiro Leme, Adriana Franco Paes Mieczkowski, Piotr Pereira, Gonçalo Amarante Guimarães BMC Genomics Research Article BACKGROUND: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. RESULTS: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC–MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. CONCLUSION: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-4440-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-17 /pmc/articles/PMC5773145/ /pubmed/29343217 http://dx.doi.org/10.1186/s12864-018-4440-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Molano, Eddy Patricia Lopez
Cabrera, Odalys García
Jose, Juliana
do Nascimento, Leandro Costa
Carazzolle, Marcelo Falsarella
Teixeira, Paulo José Pereira Lima
Alvarez, Javier Correa
Tiburcio, Ricardo Augusto
Tokimatu Filho, Paulo Massanari
de Lima, Gustavo Machado Alvares
Guido, Rafael Victório Carvalho
Corrêa, Thamy Lívia Ribeiro
Leme, Adriana Franco Paes
Mieczkowski, Piotr
Pereira, Gonçalo Amarante Guimarães
Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
title Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
title_full Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
title_fullStr Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
title_full_unstemmed Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
title_short Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)
title_sort ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-c genes (pi-plc)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773145/
https://www.ncbi.nlm.nih.gov/pubmed/29343217
http://dx.doi.org/10.1186/s12864-018-4440-4
work_keys_str_mv AT molanoeddypatricialopez ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT cabreraodalysgarcia ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT josejuliana ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT donascimentoleandrocosta ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT carazzollemarcelofalsarella ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT teixeirapaulojosepereiralima ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT alvarezjaviercorrea ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT tiburcioricardoaugusto ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT tokimatufilhopaulomassanari ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT delimagustavomachadoalvares ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT guidorafaelvictoriocarvalho ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT correathamyliviaribeiro ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT lemeadrianafrancopaes ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT mieczkowskipiotr ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc
AT pereiragoncaloamaranteguimaraes ceratocystiscacaofunestagenomeanalysisrevealsalargeexpansionofextracellularphosphatidylinositolspecificphospholipasecgenespiplc