Acute multi-sgRNA knockdown of KEOPS complex genes reproduces the microcephaly phenotype of the stable knockout zebrafish model

Until recently, morpholino oligonucleotides have been widely employed in zebrafish as an acute and efficient loss-of-function assay. However, off-target effects and reproducibility issues when compared to stable knockout lines have compromised their further use. Here we employed an acute CRISPR/Cas...

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Detalles Bibliográficos
Autores principales: Jobst-Schwan, Tilman, Schmidt, Johanna Magdalena, Schneider, Ronen, Hoogstraten, Charlotte A., Ullmann, Jeremy F. P., Schapiro, David, Majmundar, Amar J., Kolb, Amy, Eddy, Kaitlyn, Shril, Shirlee, Braun, Daniela A., Poduri, Annapurna, Hildebrandt, Friedhelm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773193/
https://www.ncbi.nlm.nih.gov/pubmed/29346415
http://dx.doi.org/10.1371/journal.pone.0191503

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