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Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats
Daikenchuto (DKT), a traditional Japanese medicine, is widely used to treat various gastrointestinal disorders. This study aimed to investigate whether DKT could promote the anastomotic healing in a rat model. Pedicled colonic segments were made in left colon by ligation of the feeding arteries, and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773623/ https://www.ncbi.nlm.nih.gov/pubmed/29348453 http://dx.doi.org/10.1038/s41598-018-19550-4 |
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author | Wada, Toshiaki Kawada, Kenji Hirai, Kenjiro Toda, Kosuke Iwamoto, Masayoshi Hasegawa, Suguru Sakai, Yoshiharu |
author_facet | Wada, Toshiaki Kawada, Kenji Hirai, Kenjiro Toda, Kosuke Iwamoto, Masayoshi Hasegawa, Suguru Sakai, Yoshiharu |
author_sort | Wada, Toshiaki |
collection | PubMed |
description | Daikenchuto (DKT), a traditional Japanese medicine, is widely used to treat various gastrointestinal disorders. This study aimed to investigate whether DKT could promote the anastomotic healing in a rat model. Pedicled colonic segments were made in left colon by ligation of the feeding arteries, and then intestinal continuity was restored. Colonic blood flow was analyzed by using ICG fluorescence imaging: Fmax, Tmax, T1/2, and Slope were calculated. Anastomotic leakage (AL) was found in 6 of 19 rats (31.6%) in the control group, whereas in 1 of 16 rats (6.2%) in the DKT group. The Fmax and Slope of DKT group were significantly higher than those of control group. DKT could promote the anastomotic healing, with the higher bursting pressure on postoperative day (POD) 2 and 5, the larger granulation thickness on POD 5, and neoangiogenesis on POD 5. Histological examination showed DKT exhibited a decreased inflammatory cell infiltration, enhanced fibroblast infiltration, and enhanced collagen density on POD 5. In the DKT group, the levels of TGFβ1 on POD 2 and VEGFα on POD5 were significantly higher, whereas the level of TNFα on POD 2 was significantly lower. Therefore, DKT could be effective for the prevention of AL following colorectal surgery. |
format | Online Article Text |
id | pubmed-5773623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57736232018-01-26 Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats Wada, Toshiaki Kawada, Kenji Hirai, Kenjiro Toda, Kosuke Iwamoto, Masayoshi Hasegawa, Suguru Sakai, Yoshiharu Sci Rep Article Daikenchuto (DKT), a traditional Japanese medicine, is widely used to treat various gastrointestinal disorders. This study aimed to investigate whether DKT could promote the anastomotic healing in a rat model. Pedicled colonic segments were made in left colon by ligation of the feeding arteries, and then intestinal continuity was restored. Colonic blood flow was analyzed by using ICG fluorescence imaging: Fmax, Tmax, T1/2, and Slope were calculated. Anastomotic leakage (AL) was found in 6 of 19 rats (31.6%) in the control group, whereas in 1 of 16 rats (6.2%) in the DKT group. The Fmax and Slope of DKT group were significantly higher than those of control group. DKT could promote the anastomotic healing, with the higher bursting pressure on postoperative day (POD) 2 and 5, the larger granulation thickness on POD 5, and neoangiogenesis on POD 5. Histological examination showed DKT exhibited a decreased inflammatory cell infiltration, enhanced fibroblast infiltration, and enhanced collagen density on POD 5. In the DKT group, the levels of TGFβ1 on POD 2 and VEGFα on POD5 were significantly higher, whereas the level of TNFα on POD 2 was significantly lower. Therefore, DKT could be effective for the prevention of AL following colorectal surgery. Nature Publishing Group UK 2018-01-18 /pmc/articles/PMC5773623/ /pubmed/29348453 http://dx.doi.org/10.1038/s41598-018-19550-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wada, Toshiaki Kawada, Kenji Hirai, Kenjiro Toda, Kosuke Iwamoto, Masayoshi Hasegawa, Suguru Sakai, Yoshiharu Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats |
title | Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats |
title_full | Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats |
title_fullStr | Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats |
title_full_unstemmed | Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats |
title_short | Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats |
title_sort | enhanced anastomotic healing by daikenchuto (tj-100) in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773623/ https://www.ncbi.nlm.nih.gov/pubmed/29348453 http://dx.doi.org/10.1038/s41598-018-19550-4 |
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