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P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples

BACKGROUND: Colorectal cancer (CRC) is one of the most common neoplasms with high mortality at advanced stages worldwide. Thus diagnosis of CRC at an early stage with sensitive molecular methods is a high priority. The aim of this study was to evaluate P16(ink4a) subcellular expression patterns in c...

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Autores principales: Gorgich, Enam Alhagh Charkhat, Heidari, Zahra, Mahmoudzadeh-Sagheb, Hamidreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773790/
https://www.ncbi.nlm.nih.gov/pubmed/29172278
http://dx.doi.org/10.22034/APJCP.2017.18.11.3049
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author Gorgich, Enam Alhagh Charkhat
Heidari, Zahra
Mahmoudzadeh-Sagheb, Hamidreza
author_facet Gorgich, Enam Alhagh Charkhat
Heidari, Zahra
Mahmoudzadeh-Sagheb, Hamidreza
author_sort Gorgich, Enam Alhagh Charkhat
collection PubMed
description BACKGROUND: Colorectal cancer (CRC) is one of the most common neoplasms with high mortality at advanced stages worldwide. Thus diagnosis of CRC at an early stage with sensitive molecular methods is a high priority. The aim of this study was to evaluate P16(ink4a) subcellular expression patterns in colorectal adenocarcinoma, adenoma and non-neoplastic tissue samples. METHODS: A total of 137 colorectal formalin fixed paraffin-embedded tissue blocks from the pathology archives of Ali-Ebne-Abitaleb central hospital, Zahedan, Iran, were examined in three groups: adenocarcinoma (n= 63), adenoma (n= 38) and non-neoplastic (n= 36). The subcellular expression pattern was determined by immunocytochemistry. Data analysis was performed using Kruskal-Wallis and Fisher exact tests with the significance level set as p<0.05. RESULTS: P16(ink4a) subcellular localization was observed in three different patterns, nuclear+cytoplasmic (73.33%), cytoplasmic (13.33%) and nuclear (13.33%). In most samples, nuclear+cytoplasmic was the predominant subcellular pattern. However, a significant difference in P16(ink4a) subcellular expression patterns was observed along the non-neoplastic, adenoma, adenocarcinoma sequence (p<0.001). An association with the histological tumor type was also noted (p=0.021). CONCLUSION: Considering variation in localization of P16(ink4a) under different pathological conditions, P16(ink4a) night be sensitive prognostic biomarker for benign colon lesions. Its use may improve strategies for screening, prognostic assessment and management of patients with CRC. Further studies are recommended in this field.
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spelling pubmed-57737902018-02-01 P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples Gorgich, Enam Alhagh Charkhat Heidari, Zahra Mahmoudzadeh-Sagheb, Hamidreza Asian Pac J Cancer Prev Research Article BACKGROUND: Colorectal cancer (CRC) is one of the most common neoplasms with high mortality at advanced stages worldwide. Thus diagnosis of CRC at an early stage with sensitive molecular methods is a high priority. The aim of this study was to evaluate P16(ink4a) subcellular expression patterns in colorectal adenocarcinoma, adenoma and non-neoplastic tissue samples. METHODS: A total of 137 colorectal formalin fixed paraffin-embedded tissue blocks from the pathology archives of Ali-Ebne-Abitaleb central hospital, Zahedan, Iran, were examined in three groups: adenocarcinoma (n= 63), adenoma (n= 38) and non-neoplastic (n= 36). The subcellular expression pattern was determined by immunocytochemistry. Data analysis was performed using Kruskal-Wallis and Fisher exact tests with the significance level set as p<0.05. RESULTS: P16(ink4a) subcellular localization was observed in three different patterns, nuclear+cytoplasmic (73.33%), cytoplasmic (13.33%) and nuclear (13.33%). In most samples, nuclear+cytoplasmic was the predominant subcellular pattern. However, a significant difference in P16(ink4a) subcellular expression patterns was observed along the non-neoplastic, adenoma, adenocarcinoma sequence (p<0.001). An association with the histological tumor type was also noted (p=0.021). CONCLUSION: Considering variation in localization of P16(ink4a) under different pathological conditions, P16(ink4a) night be sensitive prognostic biomarker for benign colon lesions. Its use may improve strategies for screening, prognostic assessment and management of patients with CRC. Further studies are recommended in this field. West Asia Organization for Cancer Prevention 2017 /pmc/articles/PMC5773790/ /pubmed/29172278 http://dx.doi.org/10.22034/APJCP.2017.18.11.3049 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Gorgich, Enam Alhagh Charkhat
Heidari, Zahra
Mahmoudzadeh-Sagheb, Hamidreza
P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
title P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
title_full P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
title_fullStr P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
title_full_unstemmed P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
title_short P16(ink4a) Subcellular Expression Patterns in Colorectal Adenocarcinoma, Adenoma and Non-Neoplastic Tissue Samples
title_sort p16(ink4a) subcellular expression patterns in colorectal adenocarcinoma, adenoma and non-neoplastic tissue samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773790/
https://www.ncbi.nlm.nih.gov/pubmed/29172278
http://dx.doi.org/10.22034/APJCP.2017.18.11.3049
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