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Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease

The autoantibodies profile of Behçet’s disease (BD) is yet incompletely understood. Annexins are a family of highly conserved proteins which are involved in some human autoimmune diseases. Autoantibodies directed toward Annexin A1 and A2 are involved in BD pathology, but correlation in their clinica...

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Autores principales: Hussain, Muhammad, Chen, Peng, Mei, Guang, Li, Yongzhe, Du, Hongwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773811/
https://www.ncbi.nlm.nih.gov/pubmed/28963375
http://dx.doi.org/10.1042/BSR20170778
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author Hussain, Muhammad
Chen, Peng
Mei, Guang
Li, Yongzhe
Du, Hongwu
author_facet Hussain, Muhammad
Chen, Peng
Mei, Guang
Li, Yongzhe
Du, Hongwu
author_sort Hussain, Muhammad
collection PubMed
description The autoantibodies profile of Behçet’s disease (BD) is yet incompletely understood. Annexins are a family of highly conserved proteins which are involved in some human autoimmune diseases. Autoantibodies directed toward Annexin A1 and A2 are involved in BD pathology, but correlation in their clinical role is controversial. The aim of our study is to estimate and evaluate the expression correlation between Annexin A1 and A2 autoantibodies in BD patients. We have designed and implemented different technical approaches to prove the hypothesis. First, bioinformatics tools such as amino acid sequence alignment, epitope prediction analysis, and 3D structural comparison were performed to find out the correlation between Annexin A1 and A2. Second, amplification of the corresponding gene by RT-PCR, then cloning, and purification techniques were applied to acquire the recombinant Annexin A1. Third, the target protein band was excised from gel electrophoresis, digested with trypsin, and analyzed by MALDI-TOF/TOF. Finally, in-house ELISA was developed to determine the induced anti-Annexin A1 autoantibodies in BD patients. Obtained results demonstrated that the BD serum reactivity against recombinant Annexin A1 was significantly higher as compared with healthy control (HC) (P<0.001). Moreover, bioassay results of Annexin A1 and A2 also showed the presence, absence, and independent coexistence of autoantibodies, when reacted with BD sera. In conclusion, Annexin A1 has a similar immunogenic expression and correlation with its analog Annexin A2 and their association may be a novel immune target of BD in Han Chinese population.
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spelling pubmed-57738112018-02-01 Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease Hussain, Muhammad Chen, Peng Mei, Guang Li, Yongzhe Du, Hongwu Biosci Rep Research Articles The autoantibodies profile of Behçet’s disease (BD) is yet incompletely understood. Annexins are a family of highly conserved proteins which are involved in some human autoimmune diseases. Autoantibodies directed toward Annexin A1 and A2 are involved in BD pathology, but correlation in their clinical role is controversial. The aim of our study is to estimate and evaluate the expression correlation between Annexin A1 and A2 autoantibodies in BD patients. We have designed and implemented different technical approaches to prove the hypothesis. First, bioinformatics tools such as amino acid sequence alignment, epitope prediction analysis, and 3D structural comparison were performed to find out the correlation between Annexin A1 and A2. Second, amplification of the corresponding gene by RT-PCR, then cloning, and purification techniques were applied to acquire the recombinant Annexin A1. Third, the target protein band was excised from gel electrophoresis, digested with trypsin, and analyzed by MALDI-TOF/TOF. Finally, in-house ELISA was developed to determine the induced anti-Annexin A1 autoantibodies in BD patients. Obtained results demonstrated that the BD serum reactivity against recombinant Annexin A1 was significantly higher as compared with healthy control (HC) (P<0.001). Moreover, bioassay results of Annexin A1 and A2 also showed the presence, absence, and independent coexistence of autoantibodies, when reacted with BD sera. In conclusion, Annexin A1 has a similar immunogenic expression and correlation with its analog Annexin A2 and their association may be a novel immune target of BD in Han Chinese population. Portland Press Ltd. 2018-01-19 /pmc/articles/PMC5773811/ /pubmed/28963375 http://dx.doi.org/10.1042/BSR20170778 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Hussain, Muhammad
Chen, Peng
Mei, Guang
Li, Yongzhe
Du, Hongwu
Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease
title Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease
title_full Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease
title_fullStr Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease
title_full_unstemmed Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease
title_short Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease
title_sort expression correlates between annexin a1 and a2 autoantibodies in patients with behçet’s disease
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773811/
https://www.ncbi.nlm.nih.gov/pubmed/28963375
http://dx.doi.org/10.1042/BSR20170778
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