Defining standard enzymatic dissociation methods for individual brains and spinal cords in EAE

OBJECTIVE: To determine the capacity, effectiveness, efficiency, and reliability of select tissue dissociation methods to isolate mononuclear cells from the CNS of mice with experimental autoimmune encephalomyelitis (EAE). METHODS: As part of an assay qualification, we tested the isolation method Pe...

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Detalles Bibliográficos
Autores principales: Hussain, Rehana Z., Miller-Little, William A., Doelger, Richard, Cutter, Gary R., Loof, Nicolas, Cravens, Petra D., Stüve, Olaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773844/
https://www.ncbi.nlm.nih.gov/pubmed/29359175
http://dx.doi.org/10.1212/NXI.0000000000000437

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773844/
https://www.ncbi.nlm.nih.gov/pubmed/29359175
http://dx.doi.org/10.1212/NXI.0000000000000437

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