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Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm

BACKGROUND: The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introdu...

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Detalles Bibliográficos
Autores principales:	Zhou, Hong, Zhou, Michael, Li, Daisy, Manthey, Joseph, Lioutikova, Ekaterina, Wang, Hong, Zeng, Xiao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5773898/ https://www.ncbi.nlm.nih.gov/pubmed/29219081 http://dx.doi.org/10.1186/s12864-017-4225-1

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