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Gibson Deletion: a novel application of isothermal in vitro recombination

BACKGROUND: Recombinant DNA technology is today a fundamental tool for virtually all biological research fields. Among the many techniques available for the construction of a “custom DNA” molecule, the isothermal in vitro assembly, or Gibson assembly, allows for an efficient, one-step, scarless reco...

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Detalles Bibliográficos
Autores principales: Kalva, Swara, Boeke, Jef D., Mita, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774033/
https://www.ncbi.nlm.nih.gov/pubmed/29375275
http://dx.doi.org/10.1186/s12575-018-0068-7