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Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection

OBJECTIVES: In a polio-free world there might be reduced funding for poliovirus surveillance. There is therefore the need to ensure that enterovirologist globally, especially those outside the global polio laboratory network, can participate in poliovirus surveillance without neglecting their entero...

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Autores principales: Faleye, Temitope Oluwasegun Cephas, Adewumi, Moses Olubusuyi, Ozegbe, Naomi Princess, Ogunsakin, Oluwaseun Elijah, Ariyo, Grace, Adeshina, Faith Wuraola, Ogunga, Oluwaseun Sarah, Oluwadare, Similoluwa Deborah, Adeniji, Johnson Adekunle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774100/
https://www.ncbi.nlm.nih.gov/pubmed/29347972
http://dx.doi.org/10.1186/s13104-018-3155-6
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author Faleye, Temitope Oluwasegun Cephas
Adewumi, Moses Olubusuyi
Ozegbe, Naomi Princess
Ogunsakin, Oluwaseun Elijah
Ariyo, Grace
Adeshina, Faith Wuraola
Ogunga, Oluwaseun Sarah
Oluwadare, Similoluwa Deborah
Adeniji, Johnson Adekunle
author_facet Faleye, Temitope Oluwasegun Cephas
Adewumi, Moses Olubusuyi
Ozegbe, Naomi Princess
Ogunsakin, Oluwaseun Elijah
Ariyo, Grace
Adeshina, Faith Wuraola
Ogunga, Oluwaseun Sarah
Oluwadare, Similoluwa Deborah
Adeniji, Johnson Adekunle
author_sort Faleye, Temitope Oluwasegun Cephas
collection PubMed
description OBJECTIVES: In a polio-free world there might be reduced funding for poliovirus surveillance. There is therefore the need to ensure that enterovirologist globally, especially those outside the global polio laboratory network, can participate in poliovirus surveillance without neglecting their enterovirus type of interest. To accomplish this, assays are needed that allow such active participation. RESULTS: In this study we describes a sensitive and specific utility extension of the recently recommended WHO RT-snPCR assay that enables independent detection of the three poliovirus types especially in cases of co-infection. More importantly, it piggy-backs on the first round PCR product of the WHO recommended assay and consequently ensures that enterovirologists interested in nonpolio enteroviruses can continue their investigations, and contribute significantly and specifically to poliovirus surveillance, by using the excess of their first round PCR product. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3155-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-57741002018-01-26 Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection Faleye, Temitope Oluwasegun Cephas Adewumi, Moses Olubusuyi Ozegbe, Naomi Princess Ogunsakin, Oluwaseun Elijah Ariyo, Grace Adeshina, Faith Wuraola Ogunga, Oluwaseun Sarah Oluwadare, Similoluwa Deborah Adeniji, Johnson Adekunle BMC Res Notes Research Note OBJECTIVES: In a polio-free world there might be reduced funding for poliovirus surveillance. There is therefore the need to ensure that enterovirologist globally, especially those outside the global polio laboratory network, can participate in poliovirus surveillance without neglecting their enterovirus type of interest. To accomplish this, assays are needed that allow such active participation. RESULTS: In this study we describes a sensitive and specific utility extension of the recently recommended WHO RT-snPCR assay that enables independent detection of the three poliovirus types especially in cases of co-infection. More importantly, it piggy-backs on the first round PCR product of the WHO recommended assay and consequently ensures that enterovirologists interested in nonpolio enteroviruses can continue their investigations, and contribute significantly and specifically to poliovirus surveillance, by using the excess of their first round PCR product. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3155-6) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-18 /pmc/articles/PMC5774100/ /pubmed/29347972 http://dx.doi.org/10.1186/s13104-018-3155-6 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Faleye, Temitope Oluwasegun Cephas
Adewumi, Moses Olubusuyi
Ozegbe, Naomi Princess
Ogunsakin, Oluwaseun Elijah
Ariyo, Grace
Adeshina, Faith Wuraola
Ogunga, Oluwaseun Sarah
Oluwadare, Similoluwa Deborah
Adeniji, Johnson Adekunle
Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
title Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
title_full Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
title_fullStr Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
title_full_unstemmed Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
title_short Extending the utility of the WHO recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
title_sort extending the utility of the who recommended assay for direct detection of enteroviruses from clinical specimen for resolving poliovirus co-infection
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774100/
https://www.ncbi.nlm.nih.gov/pubmed/29347972
http://dx.doi.org/10.1186/s13104-018-3155-6
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