Cargando…
Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms
BACKGROUND: Lactobacillus pentosus SLC13 is a high exopolysaccharide (EPS)-producing strain with broad-spectrum antimicrobial activity and the ability to grow in simulated gastrointestinal conditions. SLC13 was isolated from mustard pickles in Taiwan for potential probiotic applications. To better u...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774169/ https://www.ncbi.nlm.nih.gov/pubmed/29375672 http://dx.doi.org/10.1186/s13099-018-0228-y |
_version_ | 1783293717694644224 |
---|---|
author | Huang, Min-Lang Huang, Jing-Yao Kao, Cheng-Yen Fang, Tony J. |
author_facet | Huang, Min-Lang Huang, Jing-Yao Kao, Cheng-Yen Fang, Tony J. |
author_sort | Huang, Min-Lang |
collection | PubMed |
description | BACKGROUND: Lactobacillus pentosus SLC13 is a high exopolysaccharide (EPS)-producing strain with broad-spectrum antimicrobial activity and the ability to grow in simulated gastrointestinal conditions. SLC13 was isolated from mustard pickles in Taiwan for potential probiotic applications. To better understand the molecular base for its antimicrobial activity and high EPS production, entire genome of SLC13 was determined by PacBio SMRT sequencing. RESULTS: L. pentosus SLC13 contains a genome with a 3,520,510-bp chromosome and a 62,498-bp plasmid. GC content of the complete genome was 46.5% and that of plasmid pSLC13 was 41.3%. Sequences were annotated at the RAST prokaryotic genome annotation server, and the results showed that the genome contained 3172 coding sequences and 82 RNA genes. Seventy-six protein-coding sequences were identified on the plasmid pSLC13. A plantaricin gene cluster, which is responsible for bacteriosins biosynthesis and could be associated with its broad-spectrum antimicrobial activity, was identified based on comparative genomic analysis. Two gene clusters involved in EPS production were also identified. CONCLUSION: This genomic sequence might contribute to a future application of this strain as probiotic in productive livestock potentially inhibiting competing and pathogenic organisms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0228-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5774169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57741692018-01-26 Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms Huang, Min-Lang Huang, Jing-Yao Kao, Cheng-Yen Fang, Tony J. Gut Pathog Genome Report BACKGROUND: Lactobacillus pentosus SLC13 is a high exopolysaccharide (EPS)-producing strain with broad-spectrum antimicrobial activity and the ability to grow in simulated gastrointestinal conditions. SLC13 was isolated from mustard pickles in Taiwan for potential probiotic applications. To better understand the molecular base for its antimicrobial activity and high EPS production, entire genome of SLC13 was determined by PacBio SMRT sequencing. RESULTS: L. pentosus SLC13 contains a genome with a 3,520,510-bp chromosome and a 62,498-bp plasmid. GC content of the complete genome was 46.5% and that of plasmid pSLC13 was 41.3%. Sequences were annotated at the RAST prokaryotic genome annotation server, and the results showed that the genome contained 3172 coding sequences and 82 RNA genes. Seventy-six protein-coding sequences were identified on the plasmid pSLC13. A plantaricin gene cluster, which is responsible for bacteriosins biosynthesis and could be associated with its broad-spectrum antimicrobial activity, was identified based on comparative genomic analysis. Two gene clusters involved in EPS production were also identified. CONCLUSION: This genomic sequence might contribute to a future application of this strain as probiotic in productive livestock potentially inhibiting competing and pathogenic organisms. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0228-y) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-18 /pmc/articles/PMC5774169/ /pubmed/29375672 http://dx.doi.org/10.1186/s13099-018-0228-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Genome Report Huang, Min-Lang Huang, Jing-Yao Kao, Cheng-Yen Fang, Tony J. Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
title | Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
title_full | Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
title_fullStr | Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
title_full_unstemmed | Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
title_short | Complete genome sequence of Lactobacillus pentosus SLC13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
title_sort | complete genome sequence of lactobacillus pentosus slc13, isolated from mustard pickles, a potential probiotic strain with antimicrobial activity against foodborne pathogenic microorganisms |
topic | Genome Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774169/ https://www.ncbi.nlm.nih.gov/pubmed/29375672 http://dx.doi.org/10.1186/s13099-018-0228-y |
work_keys_str_mv | AT huangminlang completegenomesequenceoflactobacilluspentosusslc13isolatedfrommustardpicklesapotentialprobioticstrainwithantimicrobialactivityagainstfoodbornepathogenicmicroorganisms AT huangjingyao completegenomesequenceoflactobacilluspentosusslc13isolatedfrommustardpicklesapotentialprobioticstrainwithantimicrobialactivityagainstfoodbornepathogenicmicroorganisms AT kaochengyen completegenomesequenceoflactobacilluspentosusslc13isolatedfrommustardpicklesapotentialprobioticstrainwithantimicrobialactivityagainstfoodbornepathogenicmicroorganisms AT fangtonyj completegenomesequenceoflactobacilluspentosusslc13isolatedfrommustardpicklesapotentialprobioticstrainwithantimicrobialactivityagainstfoodbornepathogenicmicroorganisms |