Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection

Identifying genes required by pathogens during infection is critical for antimicrobial development. Here, we used a Monte Carlo simulation-based method to analyze high-throughput transposon sequencing data to determine the role of infection site and co-infecting microbes on the in vivo ‘essential’ g...

Descripción completa

Detalles Bibliográficos
Autores principales: Ibberson, Carolyn B., Stacy, Apollo, Fleming, Derek, Dees, Justine L., Rumbaugh, Kendra, Gilmore, Michael S., Whiteley, Marvin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774221/
https://www.ncbi.nlm.nih.gov/pubmed/28555625
http://dx.doi.org/10.1038/nmicrobiol.2017.79
_version_ 1783293721978077184
author Ibberson, Carolyn B.
Stacy, Apollo
Fleming, Derek
Dees, Justine L.
Rumbaugh, Kendra
Gilmore, Michael S.
Whiteley, Marvin
author_facet Ibberson, Carolyn B.
Stacy, Apollo
Fleming, Derek
Dees, Justine L.
Rumbaugh, Kendra
Gilmore, Michael S.
Whiteley, Marvin
author_sort Ibberson, Carolyn B.
collection PubMed
description Identifying genes required by pathogens during infection is critical for antimicrobial development. Here, we used a Monte Carlo simulation-based method to analyze high-throughput transposon sequencing data to determine the role of infection site and co-infecting microbes on the in vivo ‘essential’ genome of Staphylococcus aureus. We discovered that co-infection of murine surgical wounds with Pseudomonas aeruginosa results in conversion of ~25% of the in vivo S. aureus mono-culture essential genes to non-essential. Furthermore, 182 S. aureus genes are uniquely essential during co-infection. These “Community Dependent Essential” (CoDE) genes illustrate the importance of studying pathogen gene essentiality in polymicrobial communities.
format Online
Article
Text
id pubmed-5774221
institution National Center for Biotechnology Information
language English
publishDate 2017
record_format MEDLINE/PubMed
spelling pubmed-57742212018-01-19 Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection Ibberson, Carolyn B. Stacy, Apollo Fleming, Derek Dees, Justine L. Rumbaugh, Kendra Gilmore, Michael S. Whiteley, Marvin Nat Microbiol Article Identifying genes required by pathogens during infection is critical for antimicrobial development. Here, we used a Monte Carlo simulation-based method to analyze high-throughput transposon sequencing data to determine the role of infection site and co-infecting microbes on the in vivo ‘essential’ genome of Staphylococcus aureus. We discovered that co-infection of murine surgical wounds with Pseudomonas aeruginosa results in conversion of ~25% of the in vivo S. aureus mono-culture essential genes to non-essential. Furthermore, 182 S. aureus genes are uniquely essential during co-infection. These “Community Dependent Essential” (CoDE) genes illustrate the importance of studying pathogen gene essentiality in polymicrobial communities. 2017-05-30 /pmc/articles/PMC5774221/ /pubmed/28555625 http://dx.doi.org/10.1038/nmicrobiol.2017.79 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ibberson, Carolyn B.
Stacy, Apollo
Fleming, Derek
Dees, Justine L.
Rumbaugh, Kendra
Gilmore, Michael S.
Whiteley, Marvin
Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
title Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
title_full Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
title_fullStr Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
title_full_unstemmed Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
title_short Co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
title_sort co-infecting microbes dramatically alter pathogen gene essentiality during polymicrobial infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774221/
https://www.ncbi.nlm.nih.gov/pubmed/28555625
http://dx.doi.org/10.1038/nmicrobiol.2017.79
work_keys_str_mv AT ibbersoncarolynb coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection
AT stacyapollo coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection
AT flemingderek coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection
AT deesjustinel coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection
AT rumbaughkendra coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection
AT gilmoremichaels coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection
AT whiteleymarvin coinfectingmicrobesdramaticallyalterpathogengeneessentialityduringpolymicrobialinfection

Ejemplares similares