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Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India

BACKGROUND: The control and prevention of dengue largely depends on vector control measures, environmental management, and personal protection. Dengue control programmes are facing great challenges due to development of insecticide resistance among vector mosquitoes. Information on susceptibility st...

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Autores principales: Chatterjee, Moytrey, Ballav, Sudeep, Maji, Ardhendu K., Basu, Nandita, Sarkar, Biplab Chandra, Saha, Pabitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774824/
https://www.ncbi.nlm.nih.gov/pubmed/29309419
http://dx.doi.org/10.1371/journal.pntd.0006192
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author Chatterjee, Moytrey
Ballav, Sudeep
Maji, Ardhendu K.
Basu, Nandita
Sarkar, Biplab Chandra
Saha, Pabitra
author_facet Chatterjee, Moytrey
Ballav, Sudeep
Maji, Ardhendu K.
Basu, Nandita
Sarkar, Biplab Chandra
Saha, Pabitra
author_sort Chatterjee, Moytrey
collection PubMed
description BACKGROUND: The control and prevention of dengue largely depends on vector control measures, environmental management, and personal protection. Dengue control programmes are facing great challenges due to development of insecticide resistance among vector mosquitoes. Information on susceptibility status to different insecticides is important for national programmes to formulate vector control strategies. METHODS: We have studied the larval susceptibility of Aedes albopictus to temephos and adult susceptibility to 4% DDT, 0.05% deltamethrin, and 5% malathion as per WHO protocols in the northern districts of West Bengal. Polymorphisms in the VGSC gene were studied by direct sequencing of PCR products. RESULTS: The Ae. albopictus larval population showed sensitive [Resistance Ratio (RR(99))<3] to moderate levels of resistance (5<RR(99)>10) to temephos at different study sites. Adult bioassay results revealed that Ae. albopictus was highly resistant to DDT [Corrected Mortality (CM) < 90%] in all the study sites and susceptible to deltamethrin and malathion (CM > 98%), except in Dhupguri where a low level of resistance to deltamethrin (CM = 96.25%) was recorded. None of the six important kdr mutations (S953P, I975M/V, L978, V980G, F1474C, D1703Y) were found in the VGSC of studied mosquitoes, but we identified 11 synonymous and 1 non-synonymous mutation in the VGSC gene. CONCLUSION: The higher susceptibility level to deltamethrin and malathion, along with the absence of important kdr mutations indicates that these two insecticides are still effective against Ae. albopictus in the study areas. The susceptibility status of temephos should be monitored closely as low to moderate levels of resistance were observed in few sites. A similar study is recommended for monitoring and early detection of insecticide resistance in other parts of the country.
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spelling pubmed-57748242018-02-05 Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India Chatterjee, Moytrey Ballav, Sudeep Maji, Ardhendu K. Basu, Nandita Sarkar, Biplab Chandra Saha, Pabitra PLoS Negl Trop Dis Research Article BACKGROUND: The control and prevention of dengue largely depends on vector control measures, environmental management, and personal protection. Dengue control programmes are facing great challenges due to development of insecticide resistance among vector mosquitoes. Information on susceptibility status to different insecticides is important for national programmes to formulate vector control strategies. METHODS: We have studied the larval susceptibility of Aedes albopictus to temephos and adult susceptibility to 4% DDT, 0.05% deltamethrin, and 5% malathion as per WHO protocols in the northern districts of West Bengal. Polymorphisms in the VGSC gene were studied by direct sequencing of PCR products. RESULTS: The Ae. albopictus larval population showed sensitive [Resistance Ratio (RR(99))<3] to moderate levels of resistance (5<RR(99)>10) to temephos at different study sites. Adult bioassay results revealed that Ae. albopictus was highly resistant to DDT [Corrected Mortality (CM) < 90%] in all the study sites and susceptible to deltamethrin and malathion (CM > 98%), except in Dhupguri where a low level of resistance to deltamethrin (CM = 96.25%) was recorded. None of the six important kdr mutations (S953P, I975M/V, L978, V980G, F1474C, D1703Y) were found in the VGSC of studied mosquitoes, but we identified 11 synonymous and 1 non-synonymous mutation in the VGSC gene. CONCLUSION: The higher susceptibility level to deltamethrin and malathion, along with the absence of important kdr mutations indicates that these two insecticides are still effective against Ae. albopictus in the study areas. The susceptibility status of temephos should be monitored closely as low to moderate levels of resistance were observed in few sites. A similar study is recommended for monitoring and early detection of insecticide resistance in other parts of the country. Public Library of Science 2018-01-08 /pmc/articles/PMC5774824/ /pubmed/29309419 http://dx.doi.org/10.1371/journal.pntd.0006192 Text en © 2018 Chatterjee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chatterjee, Moytrey
Ballav, Sudeep
Maji, Ardhendu K.
Basu, Nandita
Sarkar, Biplab Chandra
Saha, Pabitra
Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India
title Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India
title_full Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India
title_fullStr Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India
title_full_unstemmed Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India
title_short Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India
title_sort polymorphisms in voltage-gated sodium channel gene and susceptibility of aedes albopictus to insecticides in three districts of northern west bengal, india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774824/
https://www.ncbi.nlm.nih.gov/pubmed/29309419
http://dx.doi.org/10.1371/journal.pntd.0006192
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