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PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1

Pseudomonas lipases are well-studied, but few studies have examined the mechanisms of lipase expression regulation. As a global regulatory protein, PmrA controls the expression of multiple genes such as the Dot/Icm apparatus, eukaryotic-like proteins, and secreted effectors. In this study, the effec...

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Autores principales: Liu, Wu, Li, Menggang, Jiao, Liangcheng, Wang, Pengbo, Yan, Yunjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775262/
https://www.ncbi.nlm.nih.gov/pubmed/29379484
http://dx.doi.org/10.3389/fmicb.2017.02690
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author Liu, Wu
Li, Menggang
Jiao, Liangcheng
Wang, Pengbo
Yan, Yunjun
author_facet Liu, Wu
Li, Menggang
Jiao, Liangcheng
Wang, Pengbo
Yan, Yunjun
author_sort Liu, Wu
collection PubMed
description Pseudomonas lipases are well-studied, but few studies have examined the mechanisms of lipase expression regulation. As a global regulatory protein, PmrA controls the expression of multiple genes such as the Dot/Icm apparatus, eukaryotic-like proteins, and secreted effectors. In this study, the effect of PmrA on expression of the lipase lipA in Pseudomonas aeruginosa PAO1 was investigated by knocking out or overexpressing pmrA, rsmY, and rsmA. PmrA regulated the expression of lipA at both the transcriptional and translational level although translation was the pivotal regulatory mechanism for lipA expression. PmrA also regulated the expression of rsmY. Using gel mobility shift assay and pmrA/rsmY double gene knock-out model, we showed that PmrA directly bound to the promoter sequence of rsmY to regulate lipA expression. Translation of lipA was activated by the PmrA/PmrB system via RsmA. Specifically, the Shine-Dalgarno (SD) sequence located at lipA mRNA was overlapped through combination between RsmA and the AGAUGA sequence, subsequently blocking the 30S ribosomal subunit to the SD sequence, leading to translational inhibition of lipA. Transcriptional repression of RsmY initiated translation of lipA through negative translational regulation of rsmA. In conclusion, this study demonstrated that in P. aeruginosa PAO1, PmrA mainly regulated rsmY expression at a translational level to influence lipA expression. RsmY primarily activated lipA translation via negative translational regulation of rsmA.
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spelling pubmed-57752622018-01-29 PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1 Liu, Wu Li, Menggang Jiao, Liangcheng Wang, Pengbo Yan, Yunjun Front Microbiol Microbiology Pseudomonas lipases are well-studied, but few studies have examined the mechanisms of lipase expression regulation. As a global regulatory protein, PmrA controls the expression of multiple genes such as the Dot/Icm apparatus, eukaryotic-like proteins, and secreted effectors. In this study, the effect of PmrA on expression of the lipase lipA in Pseudomonas aeruginosa PAO1 was investigated by knocking out or overexpressing pmrA, rsmY, and rsmA. PmrA regulated the expression of lipA at both the transcriptional and translational level although translation was the pivotal regulatory mechanism for lipA expression. PmrA also regulated the expression of rsmY. Using gel mobility shift assay and pmrA/rsmY double gene knock-out model, we showed that PmrA directly bound to the promoter sequence of rsmY to regulate lipA expression. Translation of lipA was activated by the PmrA/PmrB system via RsmA. Specifically, the Shine-Dalgarno (SD) sequence located at lipA mRNA was overlapped through combination between RsmA and the AGAUGA sequence, subsequently blocking the 30S ribosomal subunit to the SD sequence, leading to translational inhibition of lipA. Transcriptional repression of RsmY initiated translation of lipA through negative translational regulation of rsmA. In conclusion, this study demonstrated that in P. aeruginosa PAO1, PmrA mainly regulated rsmY expression at a translational level to influence lipA expression. RsmY primarily activated lipA translation via negative translational regulation of rsmA. Frontiers Media S.A. 2018-01-15 /pmc/articles/PMC5775262/ /pubmed/29379484 http://dx.doi.org/10.3389/fmicb.2017.02690 Text en Copyright © 2018 Liu, Li, Jiao, Wang and Yan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Wu
Li, Menggang
Jiao, Liangcheng
Wang, Pengbo
Yan, Yunjun
PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1
title PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1
title_full PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1
title_fullStr PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1
title_full_unstemmed PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1
title_short PmrA/PmrB Two-Component System Regulation of lipA Expression in Pseudomonas aeruginosa PAO1
title_sort pmra/pmrb two-component system regulation of lipa expression in pseudomonas aeruginosa pao1
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775262/
https://www.ncbi.nlm.nih.gov/pubmed/29379484
http://dx.doi.org/10.3389/fmicb.2017.02690
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