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African Lineage Brucella melitensis Isolates from Omani Livestock

Brucellosis is a common livestock disease in the Middle East and North Africa, but remains poorly described in the region both genetically and epidemiologically. Traditionally found in goats and sheep, Brucella melitensis is increasingly recognized as infecting camels. Most studies of brucellosis in...

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Autores principales: Foster, Jeffrey T., Walker, Faith M., Rannals, Brandy D., Hussain, M. Hammad, Drees, Kevin P., Tiller, Rebekah V., Hoffmaster, Alex R., Al-Rawahi, Abdulmajeed, Keim, Paul, Saqib, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775276/
https://www.ncbi.nlm.nih.gov/pubmed/29379492
http://dx.doi.org/10.3389/fmicb.2017.02702
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author Foster, Jeffrey T.
Walker, Faith M.
Rannals, Brandy D.
Hussain, M. Hammad
Drees, Kevin P.
Tiller, Rebekah V.
Hoffmaster, Alex R.
Al-Rawahi, Abdulmajeed
Keim, Paul
Saqib, Muhammad
author_facet Foster, Jeffrey T.
Walker, Faith M.
Rannals, Brandy D.
Hussain, M. Hammad
Drees, Kevin P.
Tiller, Rebekah V.
Hoffmaster, Alex R.
Al-Rawahi, Abdulmajeed
Keim, Paul
Saqib, Muhammad
author_sort Foster, Jeffrey T.
collection PubMed
description Brucellosis is a common livestock disease in the Middle East and North Africa, but remains poorly described in the region both genetically and epidemiologically. Traditionally found in goats and sheep, Brucella melitensis is increasingly recognized as infecting camels. Most studies of brucellosis in camels to date have focused on serological surveys, providing only limited understanding of the molecular epidemiology of circulating strains. We genotyped B. melitensis isolates from Omani camels using whole genome SNP assays and VNTRs to provide context for regional brucellosis cases. We identified a lineage of B. melitensis circulating in camels as well as in goats, sheep, and cattle in Oman. This lineage is genetically distinct from most genotypes from the Arabian Peninsula and from isolates from much of the rest of the Middle East. We then developed diagnostic assays that rapidly identify strains from this lineage. In analyses of genotypes from throughout the region, Omani isolates were genetically most closely related to strains from brucellosis cases in humans and livestock in North Africa. Our findings suggest an African origin for B. melitensis in Oman that has likely occurred through the trade of infected livestock. Moreover, African lineages of B. melitensis appear to be undersampled and consequently are underrepresented in genetic databases for Brucella. As we begin to more fully understand global genomic diversity of B. melitensis, finding and characterizing these unique but widespread lineages is essential. We predict that increased sampling of humans and livestock in Africa will reveal little known diversity in this important zoonotic pathogen.
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spelling pubmed-57752762018-01-29 African Lineage Brucella melitensis Isolates from Omani Livestock Foster, Jeffrey T. Walker, Faith M. Rannals, Brandy D. Hussain, M. Hammad Drees, Kevin P. Tiller, Rebekah V. Hoffmaster, Alex R. Al-Rawahi, Abdulmajeed Keim, Paul Saqib, Muhammad Front Microbiol Microbiology Brucellosis is a common livestock disease in the Middle East and North Africa, but remains poorly described in the region both genetically and epidemiologically. Traditionally found in goats and sheep, Brucella melitensis is increasingly recognized as infecting camels. Most studies of brucellosis in camels to date have focused on serological surveys, providing only limited understanding of the molecular epidemiology of circulating strains. We genotyped B. melitensis isolates from Omani camels using whole genome SNP assays and VNTRs to provide context for regional brucellosis cases. We identified a lineage of B. melitensis circulating in camels as well as in goats, sheep, and cattle in Oman. This lineage is genetically distinct from most genotypes from the Arabian Peninsula and from isolates from much of the rest of the Middle East. We then developed diagnostic assays that rapidly identify strains from this lineage. In analyses of genotypes from throughout the region, Omani isolates were genetically most closely related to strains from brucellosis cases in humans and livestock in North Africa. Our findings suggest an African origin for B. melitensis in Oman that has likely occurred through the trade of infected livestock. Moreover, African lineages of B. melitensis appear to be undersampled and consequently are underrepresented in genetic databases for Brucella. As we begin to more fully understand global genomic diversity of B. melitensis, finding and characterizing these unique but widespread lineages is essential. We predict that increased sampling of humans and livestock in Africa will reveal little known diversity in this important zoonotic pathogen. Frontiers Media S.A. 2018-01-15 /pmc/articles/PMC5775276/ /pubmed/29379492 http://dx.doi.org/10.3389/fmicb.2017.02702 Text en Copyright © 2018 Foster, Walker, Rannals, Hussain, Drees, Tiller, Hoffmaster, Al-Rawahi, Keim and Saqib. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Foster, Jeffrey T.
Walker, Faith M.
Rannals, Brandy D.
Hussain, M. Hammad
Drees, Kevin P.
Tiller, Rebekah V.
Hoffmaster, Alex R.
Al-Rawahi, Abdulmajeed
Keim, Paul
Saqib, Muhammad
African Lineage Brucella melitensis Isolates from Omani Livestock
title African Lineage Brucella melitensis Isolates from Omani Livestock
title_full African Lineage Brucella melitensis Isolates from Omani Livestock
title_fullStr African Lineage Brucella melitensis Isolates from Omani Livestock
title_full_unstemmed African Lineage Brucella melitensis Isolates from Omani Livestock
title_short African Lineage Brucella melitensis Isolates from Omani Livestock
title_sort african lineage brucella melitensis isolates from omani livestock
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775276/
https://www.ncbi.nlm.nih.gov/pubmed/29379492
http://dx.doi.org/10.3389/fmicb.2017.02702
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