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Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan

OBJECTIVE: β-Lactamase-negative ampicillin-resistant Haemophilus influenzae is a common opportunistic pathogen of hospital- and community-acquired infections, harboring multiple single nucleotide polymorphisms in the ftsI gene, which codes for penicillin-binding protein-3. The objectives of this stu...

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Autores principales: Misawa, Kazuhisa, Tarumoto, Norihito, Tamura, Shinsuke, Osa, Morichika, Hamamoto, Takaaki, Yuki, Atsushi, Kouzaki, Yuji, Imai, Kazuo, Ronald, Runtuwene Lucky, Yamaguchi, Toshiyuki, Murakami, Takashi, Maesaki, Shigefumi, Suzuki, Yutaka, Kawana, Akihiko, Maeda, Takuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775570/
https://www.ncbi.nlm.nih.gov/pubmed/29352811
http://dx.doi.org/10.1186/s13104-018-3169-0
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author Misawa, Kazuhisa
Tarumoto, Norihito
Tamura, Shinsuke
Osa, Morichika
Hamamoto, Takaaki
Yuki, Atsushi
Kouzaki, Yuji
Imai, Kazuo
Ronald, Runtuwene Lucky
Yamaguchi, Toshiyuki
Murakami, Takashi
Maesaki, Shigefumi
Suzuki, Yutaka
Kawana, Akihiko
Maeda, Takuya
author_facet Misawa, Kazuhisa
Tarumoto, Norihito
Tamura, Shinsuke
Osa, Morichika
Hamamoto, Takaaki
Yuki, Atsushi
Kouzaki, Yuji
Imai, Kazuo
Ronald, Runtuwene Lucky
Yamaguchi, Toshiyuki
Murakami, Takashi
Maesaki, Shigefumi
Suzuki, Yutaka
Kawana, Akihiko
Maeda, Takuya
author_sort Misawa, Kazuhisa
collection PubMed
description OBJECTIVE: β-Lactamase-negative ampicillin-resistant Haemophilus influenzae is a common opportunistic pathogen of hospital- and community-acquired infections, harboring multiple single nucleotide polymorphisms in the ftsI gene, which codes for penicillin-binding protein-3. The objectives of this study were to perform comprehensive genetic analyses of whole regions of the penicillin-binding proteins in H. influenzae and to identify additional single nucleotide polymorphisms related to antibiotic resistance, especially to ampicillin and other cephalosporins. RESULTS: In this genome analysis of the ftsI gene in 27 strains of H. influenzae, 10 of 23 (43.5%) specimens of group III genotype β-lactamase-negative ampicillin-resistant H. influenzae were paradoxically classified as ampicillin-sensitive phenotypes. Unfortunately, we could not identify any novel mutations that were significantly associated with ampicillin minimum inhibitory concentrations in other regions of the penicillin-binding proteins, and we reconfirmed that susceptibility to β-lactam antibiotics was mainly defined by previously reported SNPs in the ftsI gene. We should also consider detailed changes in expression that lead to antibiotic resistance in the future because the acquisition of resistance to antimicrobials can be predicted by the expression levels of a small number of genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3169-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-57755702018-01-31 Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan Misawa, Kazuhisa Tarumoto, Norihito Tamura, Shinsuke Osa, Morichika Hamamoto, Takaaki Yuki, Atsushi Kouzaki, Yuji Imai, Kazuo Ronald, Runtuwene Lucky Yamaguchi, Toshiyuki Murakami, Takashi Maesaki, Shigefumi Suzuki, Yutaka Kawana, Akihiko Maeda, Takuya BMC Res Notes Research Note OBJECTIVE: β-Lactamase-negative ampicillin-resistant Haemophilus influenzae is a common opportunistic pathogen of hospital- and community-acquired infections, harboring multiple single nucleotide polymorphisms in the ftsI gene, which codes for penicillin-binding protein-3. The objectives of this study were to perform comprehensive genetic analyses of whole regions of the penicillin-binding proteins in H. influenzae and to identify additional single nucleotide polymorphisms related to antibiotic resistance, especially to ampicillin and other cephalosporins. RESULTS: In this genome analysis of the ftsI gene in 27 strains of H. influenzae, 10 of 23 (43.5%) specimens of group III genotype β-lactamase-negative ampicillin-resistant H. influenzae were paradoxically classified as ampicillin-sensitive phenotypes. Unfortunately, we could not identify any novel mutations that were significantly associated with ampicillin minimum inhibitory concentrations in other regions of the penicillin-binding proteins, and we reconfirmed that susceptibility to β-lactam antibiotics was mainly defined by previously reported SNPs in the ftsI gene. We should also consider detailed changes in expression that lead to antibiotic resistance in the future because the acquisition of resistance to antimicrobials can be predicted by the expression levels of a small number of genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3169-0) contains supplementary material, which is available to authorized users. BioMed Central 2018-01-20 /pmc/articles/PMC5775570/ /pubmed/29352811 http://dx.doi.org/10.1186/s13104-018-3169-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Misawa, Kazuhisa
Tarumoto, Norihito
Tamura, Shinsuke
Osa, Morichika
Hamamoto, Takaaki
Yuki, Atsushi
Kouzaki, Yuji
Imai, Kazuo
Ronald, Runtuwene Lucky
Yamaguchi, Toshiyuki
Murakami, Takashi
Maesaki, Shigefumi
Suzuki, Yutaka
Kawana, Akihiko
Maeda, Takuya
Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
title Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
title_full Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
title_fullStr Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
title_full_unstemmed Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
title_short Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
title_sort single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant haemophilus influenzae in japan
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775570/
https://www.ncbi.nlm.nih.gov/pubmed/29352811
http://dx.doi.org/10.1186/s13104-018-3169-0
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