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Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema
OBJECTIVE(S): Reactive oxygen species (ROS)-produced oxidative disorders were involved at the pathophysiology of many inflammatory processes via the generation of pro-inflammatory cytokines and antioxidant defense system suppression. Although herbal antioxidants as mono-therapy relief many inflammat...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776444/ https://www.ncbi.nlm.nih.gov/pubmed/29372043 http://dx.doi.org/10.22038/IJBMS.2017.25067.6219 |
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author | Abd-Allah, Amany Ahmed Mohamed El-Deen, Nasr Abdelwahab Mohamed Nasr Mohamed, Wafaa Abdou Mohamed Naguib, Fatma Mohamed |
author_facet | Abd-Allah, Amany Ahmed Mohamed El-Deen, Nasr Abdelwahab Mohamed Nasr Mohamed, Wafaa Abdou Mohamed Naguib, Fatma Mohamed |
author_sort | Abd-Allah, Amany Ahmed Mohamed |
collection | PubMed |
description | OBJECTIVE(S): Reactive oxygen species (ROS)-produced oxidative disorders were involved at the pathophysiology of many inflammatory processes via the generation of pro-inflammatory cytokines and antioxidant defense system suppression. Although herbal antioxidants as mono-therapy relief many inflammatory diseases including, autoimmunity rheumatoid arthritis, but as combination therapy with other proven anti-inflammatory drugs in order to decreasing their toxic impacts has not yet been studied clearly, especially against chemical substances that’s induced local inflammation with characteristic edema. MATERIALS AND METHODS: Grape seeds extract (GSE) at a concentration of 40 mg/kg B. wt alone or in combination with indomethacin (Indo.) at a dose of 5 mg/Kg B. wt orally given for 10 days prior (gps VI, VII, VIII) or as a single dose after edema induction (gps IX, X, XI) in rat’s left hind paw by sub-planter single injection of 0.1 carrageenan: saline solution (1%) (gp. V) to assess the prophylactic and therapeutic anti-inflammatory activities of both through the estimation of selective inflammatory mediators and oxidative damage-related biomarkers as well as tissue mast cell scoring. Furthermore, both substances were given alone (gps II, III, IV) for their blood, liver and kidney safety evaluation comparing with negative control rats (gp. I) which kept without medication. RESULTS: A marked reduction on the inflammatory mediators, edema volume and oxidative byproducts in edema bearing rats’ prophylactic and treated with grape seeds extract and indomethacin was observed. Indomethacin found to induce some toxicological impacts which minimized when administered together with GSE. CONCLUSION: GSE is a safe antioxidant agent with anti-inflammatory property. |
format | Online Article Text |
id | pubmed-5776444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-57764442018-01-25 Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema Abd-Allah, Amany Ahmed Mohamed El-Deen, Nasr Abdelwahab Mohamed Nasr Mohamed, Wafaa Abdou Mohamed Naguib, Fatma Mohamed Iran J Basic Med Sci Short Communication OBJECTIVE(S): Reactive oxygen species (ROS)-produced oxidative disorders were involved at the pathophysiology of many inflammatory processes via the generation of pro-inflammatory cytokines and antioxidant defense system suppression. Although herbal antioxidants as mono-therapy relief many inflammatory diseases including, autoimmunity rheumatoid arthritis, but as combination therapy with other proven anti-inflammatory drugs in order to decreasing their toxic impacts has not yet been studied clearly, especially against chemical substances that’s induced local inflammation with characteristic edema. MATERIALS AND METHODS: Grape seeds extract (GSE) at a concentration of 40 mg/kg B. wt alone or in combination with indomethacin (Indo.) at a dose of 5 mg/Kg B. wt orally given for 10 days prior (gps VI, VII, VIII) or as a single dose after edema induction (gps IX, X, XI) in rat’s left hind paw by sub-planter single injection of 0.1 carrageenan: saline solution (1%) (gp. V) to assess the prophylactic and therapeutic anti-inflammatory activities of both through the estimation of selective inflammatory mediators and oxidative damage-related biomarkers as well as tissue mast cell scoring. Furthermore, both substances were given alone (gps II, III, IV) for their blood, liver and kidney safety evaluation comparing with negative control rats (gp. I) which kept without medication. RESULTS: A marked reduction on the inflammatory mediators, edema volume and oxidative byproducts in edema bearing rats’ prophylactic and treated with grape seeds extract and indomethacin was observed. Indomethacin found to induce some toxicological impacts which minimized when administered together with GSE. CONCLUSION: GSE is a safe antioxidant agent with anti-inflammatory property. Mashhad University of Medical Sciences 2018-01 /pmc/articles/PMC5776444/ /pubmed/29372043 http://dx.doi.org/10.22038/IJBMS.2017.25067.6219 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Abd-Allah, Amany Ahmed Mohamed El-Deen, Nasr Abdelwahab Mohamed Nasr Mohamed, Wafaa Abdou Mohamed Naguib, Fatma Mohamed Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
title | Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
title_full | Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
title_fullStr | Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
title_full_unstemmed | Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
title_short | Mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
title_sort | mast cells and pro-inflammatory cytokines roles in assessment of grape seeds extract anti-inflammatory activity in rat model of carrageenan-induced paw edema |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776444/ https://www.ncbi.nlm.nih.gov/pubmed/29372043 http://dx.doi.org/10.22038/IJBMS.2017.25067.6219 |
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