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The genotoxicity of an aqueous extract of Gyejibokryeong-hwan

BACKGROUND: Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity of Gyejibokryeong-hwan has not yet been well established. METHODS: The present s...

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Autores principales: Lee, Mee-Young, Seo, Chang-Seob, Ha, Hyekyung, Park, Eunsook, Kim, Ji-Young, Shin, Hyeun-Kyoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776759/
https://www.ncbi.nlm.nih.gov/pubmed/29357857
http://dx.doi.org/10.1186/s12906-017-2054-z
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author Lee, Mee-Young
Seo, Chang-Seob
Ha, Hyekyung
Park, Eunsook
Kim, Ji-Young
Shin, Hyeun-Kyoo
author_facet Lee, Mee-Young
Seo, Chang-Seob
Ha, Hyekyung
Park, Eunsook
Kim, Ji-Young
Shin, Hyeun-Kyoo
author_sort Lee, Mee-Young
collection PubMed
description BACKGROUND: Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity of Gyejibokryeong-hwan has not yet been well established. METHODS: The present study investigated that the genotoxicity of an aqueous extract of Gyejibokryeong-hwan (GJBRHE): an in vitro chromosomal aberration test using Chinese hamster lung cells, an in vitro bacterial reverse mutation assay (Ames test) with Salmonella typhimurium and Escherichia coli strains, and an in vivo micronucleus test using ICR mouse bone marrow. RESULTS: GJBRHE with or without the S9 mix showed no genotoxicity in the Ames test up to 5000 μg/plate or in the in vivo MN test up to 2000 mg/kg body weight. In contrast, the chromosomal aberration test showed that GJBRHE induced an increase in the number of chromosomal aberrations compared with the control after treatment for 6 h with 4200 μg/mL GJBRHE in the presence of the S9 mix and for 22 h with 800 μg/mL GJBRHE in the absence of the S9 mix. CONCLUSIONS: GJBRHE did not cause detectable genotoxic effects in the bacterial mutation test or the in vivo MN test, however genotoxic effect was detected in the in vitro chromosomal aberration assay. Our results suggest that GJBRHE may be associated with a low risk of carcinogenesis. Thus, further detailed experiments would be needed to clarify the compound responsible for inducing this genotoxicity of GJBRHE and to determine its mechanism.
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spelling pubmed-57767592018-01-31 The genotoxicity of an aqueous extract of Gyejibokryeong-hwan Lee, Mee-Young Seo, Chang-Seob Ha, Hyekyung Park, Eunsook Kim, Ji-Young Shin, Hyeun-Kyoo BMC Complement Altern Med Research Article BACKGROUND: Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity of Gyejibokryeong-hwan has not yet been well established. METHODS: The present study investigated that the genotoxicity of an aqueous extract of Gyejibokryeong-hwan (GJBRHE): an in vitro chromosomal aberration test using Chinese hamster lung cells, an in vitro bacterial reverse mutation assay (Ames test) with Salmonella typhimurium and Escherichia coli strains, and an in vivo micronucleus test using ICR mouse bone marrow. RESULTS: GJBRHE with or without the S9 mix showed no genotoxicity in the Ames test up to 5000 μg/plate or in the in vivo MN test up to 2000 mg/kg body weight. In contrast, the chromosomal aberration test showed that GJBRHE induced an increase in the number of chromosomal aberrations compared with the control after treatment for 6 h with 4200 μg/mL GJBRHE in the presence of the S9 mix and for 22 h with 800 μg/mL GJBRHE in the absence of the S9 mix. CONCLUSIONS: GJBRHE did not cause detectable genotoxic effects in the bacterial mutation test or the in vivo MN test, however genotoxic effect was detected in the in vitro chromosomal aberration assay. Our results suggest that GJBRHE may be associated with a low risk of carcinogenesis. Thus, further detailed experiments would be needed to clarify the compound responsible for inducing this genotoxicity of GJBRHE and to determine its mechanism. BioMed Central 2018-01-22 /pmc/articles/PMC5776759/ /pubmed/29357857 http://dx.doi.org/10.1186/s12906-017-2054-z Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Lee, Mee-Young
Seo, Chang-Seob
Ha, Hyekyung
Park, Eunsook
Kim, Ji-Young
Shin, Hyeun-Kyoo
The genotoxicity of an aqueous extract of Gyejibokryeong-hwan
title The genotoxicity of an aqueous extract of Gyejibokryeong-hwan
title_full The genotoxicity of an aqueous extract of Gyejibokryeong-hwan
title_fullStr The genotoxicity of an aqueous extract of Gyejibokryeong-hwan
title_full_unstemmed The genotoxicity of an aqueous extract of Gyejibokryeong-hwan
title_short The genotoxicity of an aqueous extract of Gyejibokryeong-hwan
title_sort genotoxicity of an aqueous extract of gyejibokryeong-hwan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776759/
https://www.ncbi.nlm.nih.gov/pubmed/29357857
http://dx.doi.org/10.1186/s12906-017-2054-z
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