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Extracellular Lactate: A Novel Measure of T Cell Proliferation

Following activation, T cells rapidly divide and acquire effector functions. This energetically demanding process depends upon the ability of T cells to undergo metabolic remodeling from oxidative phosphorylation to aerobic glycolysis, during which glucose is converted into lactate and released extr...

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Autores principales: Grist, James T., Jarvis, Lorna B., Georgieva, Zoya, Thompson, Sara, Kaur Sandhu, Harpreet, Burling, Keith, Clarke, Ashley, Jackson, Sarah, Wills, Mark, Gallagher, Ferdia A., Jones, Joanne L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776880/
https://www.ncbi.nlm.nih.gov/pubmed/29288205
http://dx.doi.org/10.4049/jimmunol.1700886
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author Grist, James T.
Jarvis, Lorna B.
Georgieva, Zoya
Thompson, Sara
Kaur Sandhu, Harpreet
Burling, Keith
Clarke, Ashley
Jackson, Sarah
Wills, Mark
Gallagher, Ferdia A.
Jones, Joanne L.
author_facet Grist, James T.
Jarvis, Lorna B.
Georgieva, Zoya
Thompson, Sara
Kaur Sandhu, Harpreet
Burling, Keith
Clarke, Ashley
Jackson, Sarah
Wills, Mark
Gallagher, Ferdia A.
Jones, Joanne L.
author_sort Grist, James T.
collection PubMed
description Following activation, T cells rapidly divide and acquire effector functions. This energetically demanding process depends upon the ability of T cells to undergo metabolic remodeling from oxidative phosphorylation to aerobic glycolysis, during which glucose is converted into lactate and released extracellularly. In this article, we demonstrate that extracellular lactate can be used to dynamically assess human T cell responses in vitro. Extracellular lactate levels strongly correlated with T cell proliferation, and measuring lactate compared favorably with traditional methods for determining T cell responses (i.e., [(3)H]thymidine incorporation and the use of cell proliferation dyes). Furthermore, we demonstrate the usefulness of measuring lactate as a read-out in conventional suppression assays and high-throughput peptide-screening assays. Extracellular lactate was stably produced over 7 d, and results were reproducibly performed over several freeze–thaw cycles. We conclude that the use of extracellular lactate measurements can be a sensitive, safe, stable, and easy-to-implement research tool for measuring T cell responses and cellular metabolic changes in vitro.
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spelling pubmed-57768802018-01-24 Extracellular Lactate: A Novel Measure of T Cell Proliferation Grist, James T. Jarvis, Lorna B. Georgieva, Zoya Thompson, Sara Kaur Sandhu, Harpreet Burling, Keith Clarke, Ashley Jackson, Sarah Wills, Mark Gallagher, Ferdia A. Jones, Joanne L. J Immunol Novel Immunological Methods Following activation, T cells rapidly divide and acquire effector functions. This energetically demanding process depends upon the ability of T cells to undergo metabolic remodeling from oxidative phosphorylation to aerobic glycolysis, during which glucose is converted into lactate and released extracellularly. In this article, we demonstrate that extracellular lactate can be used to dynamically assess human T cell responses in vitro. Extracellular lactate levels strongly correlated with T cell proliferation, and measuring lactate compared favorably with traditional methods for determining T cell responses (i.e., [(3)H]thymidine incorporation and the use of cell proliferation dyes). Furthermore, we demonstrate the usefulness of measuring lactate as a read-out in conventional suppression assays and high-throughput peptide-screening assays. Extracellular lactate was stably produced over 7 d, and results were reproducibly performed over several freeze–thaw cycles. We conclude that the use of extracellular lactate measurements can be a sensitive, safe, stable, and easy-to-implement research tool for measuring T cell responses and cellular metabolic changes in vitro. AAI 2018-02-01 2017-12-29 /pmc/articles/PMC5776880/ /pubmed/29288205 http://dx.doi.org/10.4049/jimmunol.1700886 Text en Copyright © 2018 The Authors https://creativecommons.org/licenses/by/4.0 This article is distributed under the terms of the CC BY 4.0 Unported license.
spellingShingle Novel Immunological Methods
Grist, James T.
Jarvis, Lorna B.
Georgieva, Zoya
Thompson, Sara
Kaur Sandhu, Harpreet
Burling, Keith
Clarke, Ashley
Jackson, Sarah
Wills, Mark
Gallagher, Ferdia A.
Jones, Joanne L.
Extracellular Lactate: A Novel Measure of T Cell Proliferation
title Extracellular Lactate: A Novel Measure of T Cell Proliferation
title_full Extracellular Lactate: A Novel Measure of T Cell Proliferation
title_fullStr Extracellular Lactate: A Novel Measure of T Cell Proliferation
title_full_unstemmed Extracellular Lactate: A Novel Measure of T Cell Proliferation
title_short Extracellular Lactate: A Novel Measure of T Cell Proliferation
title_sort extracellular lactate: a novel measure of t cell proliferation
topic Novel Immunological Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776880/
https://www.ncbi.nlm.nih.gov/pubmed/29288205
http://dx.doi.org/10.4049/jimmunol.1700886
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