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Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages wh...

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Autores principales: Matin, Somaie, Shahbazi, Gholamreza, Namin, Shervin Tabrizian, Moradpour, Rouhallah, Feizi, Farideh, Piri-dogahe, Hadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776894/
https://www.ncbi.nlm.nih.gov/pubmed/29320815
http://dx.doi.org/10.3347/kjp.2017.55.6.607
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author Matin, Somaie
Shahbazi, Gholamreza
Namin, Shervin Tabrizian
Moradpour, Rouhallah
Feizi, Farideh
Piri-dogahe, Hadi
author_facet Matin, Somaie
Shahbazi, Gholamreza
Namin, Shervin Tabrizian
Moradpour, Rouhallah
Feizi, Farideh
Piri-dogahe, Hadi
author_sort Matin, Somaie
collection PubMed
description Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.
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spelling pubmed-57768942018-01-25 Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran Matin, Somaie Shahbazi, Gholamreza Namin, Shervin Tabrizian Moradpour, Rouhallah Feizi, Farideh Piri-dogahe, Hadi Korean J Parasitol Original Article Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity. The Korean Society for Parasitology and Tropical Medicine 2017-12 2017-12-31 /pmc/articles/PMC5776894/ /pubmed/29320815 http://dx.doi.org/10.3347/kjp.2017.55.6.607 Text en Copyright © 2017 by The Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Matin, Somaie
Shahbazi, Gholamreza
Namin, Shervin Tabrizian
Moradpour, Rouhallah
Feizi, Farideh
Piri-dogahe, Hadi
Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
title Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
title_full Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
title_fullStr Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
title_full_unstemmed Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
title_short Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran
title_sort comparison of placenta pcr and maternal serology of aborted women for detection of toxoplasma gondii in ardabil, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776894/
https://www.ncbi.nlm.nih.gov/pubmed/29320815
http://dx.doi.org/10.3347/kjp.2017.55.6.607
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