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The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue

Micro (mi)RNAs are an endogenous non-coding small RNA comprised of 19–26 nucleotides. miRNAs regulate gene expression through the recognition of its ‘seed sequence’ and interactions with 3′-untranslated region of target miRNAs. Previous studies identified that miRNAs are associated with the onset an...

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Autores principales: Zhang, Jingcheng, Wei, Bin, Hu, Huixian, Liu, Fanrong, Tu, Yan, He, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776920/
https://www.ncbi.nlm.nih.gov/pubmed/29434917
http://dx.doi.org/10.3892/ol.2017.7550
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author Zhang, Jingcheng
Wei, Bin
Hu, Huixian
Liu, Fanrong
Tu, Yan
He, Fang
author_facet Zhang, Jingcheng
Wei, Bin
Hu, Huixian
Liu, Fanrong
Tu, Yan
He, Fang
author_sort Zhang, Jingcheng
collection PubMed
description Micro (mi)RNAs are an endogenous non-coding small RNA comprised of 19–26 nucleotides. miRNAs regulate gene expression through the recognition of its ‘seed sequence’ and interactions with 3′-untranslated region of target miRNAs. Previous studies identified that miRNAs are associated with the onset and development of breast cancer and that a number of mutations in the coding DNA sequence of miRNAs affect its expression. Therefore, the present study aimed to screen differentially expressed miRNAs using miRNA expression profile chips to analyze the expression of miRNA (miR)-205 in 12 breast cell lines of different metastatic performance and benign proliferative variation as well as breast cancer via in-situ hybridization, and screen out single-nucleotide polymorphisms (SNPs) in the miR-205 coding gene region. In addition, the association between SNP and the clinicopathological features of breast lesions was investigated. The results of the present study demonstrated that the expression of miR-205 was decreased in breast cancer cells and tissues compared with benign lesions. SNPs in the miR-205 coding gene region were identified in tumor cell lines and as increasing lengths of the AGC repetitive sequence were lost, miR-205 expression increased. An association was identified between a number of SNPs in the miR-205 coding gene region and breast cancer, as well as between SNPs in miR-205 coding gene region and the clinicopathological features of breast cancer.
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spelling pubmed-57769202018-02-12 The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue Zhang, Jingcheng Wei, Bin Hu, Huixian Liu, Fanrong Tu, Yan He, Fang Oncol Lett Articles Micro (mi)RNAs are an endogenous non-coding small RNA comprised of 19–26 nucleotides. miRNAs regulate gene expression through the recognition of its ‘seed sequence’ and interactions with 3′-untranslated region of target miRNAs. Previous studies identified that miRNAs are associated with the onset and development of breast cancer and that a number of mutations in the coding DNA sequence of miRNAs affect its expression. Therefore, the present study aimed to screen differentially expressed miRNAs using miRNA expression profile chips to analyze the expression of miRNA (miR)-205 in 12 breast cell lines of different metastatic performance and benign proliferative variation as well as breast cancer via in-situ hybridization, and screen out single-nucleotide polymorphisms (SNPs) in the miR-205 coding gene region. In addition, the association between SNP and the clinicopathological features of breast lesions was investigated. The results of the present study demonstrated that the expression of miR-205 was decreased in breast cancer cells and tissues compared with benign lesions. SNPs in the miR-205 coding gene region were identified in tumor cell lines and as increasing lengths of the AGC repetitive sequence were lost, miR-205 expression increased. An association was identified between a number of SNPs in the miR-205 coding gene region and breast cancer, as well as between SNPs in miR-205 coding gene region and the clinicopathological features of breast cancer. D.A. Spandidos 2018-02 2017-12-08 /pmc/articles/PMC5776920/ /pubmed/29434917 http://dx.doi.org/10.3892/ol.2017.7550 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Jingcheng
Wei, Bin
Hu, Huixian
Liu, Fanrong
Tu, Yan
He, Fang
The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue
title The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue
title_full The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue
title_fullStr The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue
title_full_unstemmed The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue
title_short The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue
title_sort association between differentially expressed micro rnas in breast cancer cell lines and the micro rna-205 gene polymorphism in breast cancer tissue
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776920/
https://www.ncbi.nlm.nih.gov/pubmed/29434917
http://dx.doi.org/10.3892/ol.2017.7550
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