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Four-week individual caging of male ICR mice alters body composition without change in body mass

Understanding the physiological implications of caging conditions for mice is crucial in improving the replicability and reliability of animal research. Individual caging of mice is known to alter mouse psychology, such as triggering depression-like symptoms in mice, suggesting that caging condition...

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Autores principales: Shin, Jisu, Woo, Jiwan, Cho, Yakdol, Choi, Yoon Hee, Shin, Naewoo Neo, Kim, YoungSoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778080/
https://www.ncbi.nlm.nih.gov/pubmed/29358648
http://dx.doi.org/10.1038/s41598-018-19808-x
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author Shin, Jisu
Woo, Jiwan
Cho, Yakdol
Choi, Yoon Hee
Shin, Naewoo Neo
Kim, YoungSoo
author_facet Shin, Jisu
Woo, Jiwan
Cho, Yakdol
Choi, Yoon Hee
Shin, Naewoo Neo
Kim, YoungSoo
author_sort Shin, Jisu
collection PubMed
description Understanding the physiological implications of caging conditions for mice is crucial in improving the replicability and reliability of animal research. Individual caging of mice is known to alter mouse psychology, such as triggering depression-like symptoms in mice, suggesting that caging conditions could have negative effects on mice. Therefore, we hypothesized that individual caging could affect the physical composition of outbred mice. To investigate this, dual X-ray absorptiometry (DXA) was used to compare the mass, bone mineral content (BMC), bone mineral density (BMD), lean tissue percentage and fat tissue percentage between group and individual caged mice. We also conducted open field test to compare mouse activities in different caging conditions. Our results showed significantly reduced BMD and lean tissue percentage and significantly increased fat tissue percentage in individually-caged male mice. Furthermore, there were no differences in body mass and activity between the grouped and individual mice, suggesting that these physical alterations were not induced by group-related activity. In this study, we conclude that individual caging could alter the body composition of mice without affecting external morphology.
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spelling pubmed-57780802018-01-31 Four-week individual caging of male ICR mice alters body composition without change in body mass Shin, Jisu Woo, Jiwan Cho, Yakdol Choi, Yoon Hee Shin, Naewoo Neo Kim, YoungSoo Sci Rep Article Understanding the physiological implications of caging conditions for mice is crucial in improving the replicability and reliability of animal research. Individual caging of mice is known to alter mouse psychology, such as triggering depression-like symptoms in mice, suggesting that caging conditions could have negative effects on mice. Therefore, we hypothesized that individual caging could affect the physical composition of outbred mice. To investigate this, dual X-ray absorptiometry (DXA) was used to compare the mass, bone mineral content (BMC), bone mineral density (BMD), lean tissue percentage and fat tissue percentage between group and individual caged mice. We also conducted open field test to compare mouse activities in different caging conditions. Our results showed significantly reduced BMD and lean tissue percentage and significantly increased fat tissue percentage in individually-caged male mice. Furthermore, there were no differences in body mass and activity between the grouped and individual mice, suggesting that these physical alterations were not induced by group-related activity. In this study, we conclude that individual caging could alter the body composition of mice without affecting external morphology. Nature Publishing Group UK 2018-01-22 /pmc/articles/PMC5778080/ /pubmed/29358648 http://dx.doi.org/10.1038/s41598-018-19808-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shin, Jisu
Woo, Jiwan
Cho, Yakdol
Choi, Yoon Hee
Shin, Naewoo Neo
Kim, YoungSoo
Four-week individual caging of male ICR mice alters body composition without change in body mass
title Four-week individual caging of male ICR mice alters body composition without change in body mass
title_full Four-week individual caging of male ICR mice alters body composition without change in body mass
title_fullStr Four-week individual caging of male ICR mice alters body composition without change in body mass
title_full_unstemmed Four-week individual caging of male ICR mice alters body composition without change in body mass
title_short Four-week individual caging of male ICR mice alters body composition without change in body mass
title_sort four-week individual caging of male icr mice alters body composition without change in body mass
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778080/
https://www.ncbi.nlm.nih.gov/pubmed/29358648
http://dx.doi.org/10.1038/s41598-018-19808-x
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