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Method to Assemble Genomic DNA Fragments or Genes on Human Artificial Chromosome with Regulated Kinetochore Using a Multi-Integrase System

[Image: see text] The production of cells capable of carrying multiple transgenes to Mb-size genomic loci has multiple applications in biomedicine and biotechnology. In order to achieve this goal, three key steps are required: (i) cloning of large genomic segments; (ii) insertion of multiple DNA blo...

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Detalles Bibliográficos
Autores principales:	Lee, Nicholas C. O., Kim, Jung-Hyun, Petrov, Nikolai S., Lee, Hee-Sheung, Masumoto, Hiroshi, Earnshaw, William C., Larionov, Vladimir, Kouprina, Natalay
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2017
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778389/ https://www.ncbi.nlm.nih.gov/pubmed/28799737 http://dx.doi.org/10.1021/acssynbio.7b00209

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