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Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids

[Image: see text] One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple f...

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Autores principales: Exterkate, Marten, Caforio, Antonella, Stuart, Marc C. A., Driessen, Arnold J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778391/
https://www.ncbi.nlm.nih.gov/pubmed/28922922
http://dx.doi.org/10.1021/acssynbio.7b00265
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author Exterkate, Marten
Caforio, Antonella
Stuart, Marc C. A.
Driessen, Arnold J. M.
author_facet Exterkate, Marten
Caforio, Antonella
Stuart, Marc C. A.
Driessen, Arnold J. M.
author_sort Exterkate, Marten
collection PubMed
description [Image: see text] One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in acyl-chain and polar headgroup compositions. Due to the high fidelity and versatility, complete conversion of the fatty acid substrates into multiple phospholipid species is achieved simultaneously, leading to membrane expansion as a first step toward a synthetic minimal cell.
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spelling pubmed-57783912018-01-25 Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids Exterkate, Marten Caforio, Antonella Stuart, Marc C. A. Driessen, Arnold J. M. ACS Synth Biol [Image: see text] One of the key aspects that defines a cell as a living entity is its ability to self-reproduce. In this process, membrane biogenesis is an essential element. Here, we developed an in vitro phospholipid biosynthesis pathway based on a cascade of eight enzymes, starting from simple fatty acid building blocks and glycerol 3-phosphate. The reconstituted system yields multiple phospholipid species that vary in acyl-chain and polar headgroup compositions. Due to the high fidelity and versatility, complete conversion of the fatty acid substrates into multiple phospholipid species is achieved simultaneously, leading to membrane expansion as a first step toward a synthetic minimal cell. American Chemical Society 2017-09-18 2018-01-19 /pmc/articles/PMC5778391/ /pubmed/28922922 http://dx.doi.org/10.1021/acssynbio.7b00265 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Exterkate, Marten
Caforio, Antonella
Stuart, Marc C. A.
Driessen, Arnold J. M.
Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
title Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
title_full Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
title_fullStr Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
title_full_unstemmed Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
title_short Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids
title_sort growing membranes in vitro by continuous phospholipid biosynthesis from free fatty acids
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778391/
https://www.ncbi.nlm.nih.gov/pubmed/28922922
http://dx.doi.org/10.1021/acssynbio.7b00265
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