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A bacterial three-hybrid assay detects Escherichia coli Hfq–sRNA interactions in vivo
The interaction of RNA molecules with proteins is a critical aspect of gene regulation across all domains of life. Here, we report the development of a bacterial three-hybrid (B3H) assay to genetically detect RNA–protein interactions. The basis for this three-hybrid assay is a transcription-based ba...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778611/ https://www.ncbi.nlm.nih.gov/pubmed/29140461 http://dx.doi.org/10.1093/nar/gkx1086 |