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Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778793/ https://www.ncbi.nlm.nih.gov/pubmed/29357901 http://dx.doi.org/10.1186/s13071-018-2639-5 |
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author | Magnet, Angela Gomes, Thiago Santos Pardinas, Carmen Garcia de Blas, Natalia Sadaba, Cruz Carrillo, Eugenia Izquierdo, Fernando del Castillo, José Manuel Benítez Hurtado, Carolina del Aguila, Carmen Fenoy, Soledad |
author_facet | Magnet, Angela Gomes, Thiago Santos Pardinas, Carmen Garcia de Blas, Natalia Sadaba, Cruz Carrillo, Eugenia Izquierdo, Fernando del Castillo, José Manuel Benítez Hurtado, Carolina del Aguila, Carmen Fenoy, Soledad |
author_sort | Magnet, Angela |
collection | PubMed |
description | BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. METHODS: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. RESULTS: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. CONCLUSIONS: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out. |
format | Online Article Text |
id | pubmed-5778793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57787932018-01-31 Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach Magnet, Angela Gomes, Thiago Santos Pardinas, Carmen Garcia de Blas, Natalia Sadaba, Cruz Carrillo, Eugenia Izquierdo, Fernando del Castillo, José Manuel Benítez Hurtado, Carolina del Aguila, Carmen Fenoy, Soledad Parasit Vectors Research BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. METHODS: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. RESULTS: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. CONCLUSIONS: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out. BioMed Central 2018-01-22 /pmc/articles/PMC5778793/ /pubmed/29357901 http://dx.doi.org/10.1186/s13071-018-2639-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Magnet, Angela Gomes, Thiago Santos Pardinas, Carmen Garcia de Blas, Natalia Sadaba, Cruz Carrillo, Eugenia Izquierdo, Fernando del Castillo, José Manuel Benítez Hurtado, Carolina del Aguila, Carmen Fenoy, Soledad Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
title | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
title_full | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
title_fullStr | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
title_full_unstemmed | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
title_short | Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach |
title_sort | can artificial tears prevent acanthamoeba keratitis? an in vitro approach |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778793/ https://www.ncbi.nlm.nih.gov/pubmed/29357901 http://dx.doi.org/10.1186/s13071-018-2639-5 |
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