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Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach

BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the...

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Autores principales: Magnet, Angela, Gomes, Thiago Santos, Pardinas, Carmen, Garcia de Blas, Natalia, Sadaba, Cruz, Carrillo, Eugenia, Izquierdo, Fernando, del Castillo, José Manuel Benítez, Hurtado, Carolina, del Aguila, Carmen, Fenoy, Soledad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778793/
https://www.ncbi.nlm.nih.gov/pubmed/29357901
http://dx.doi.org/10.1186/s13071-018-2639-5
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author Magnet, Angela
Gomes, Thiago Santos
Pardinas, Carmen
Garcia de Blas, Natalia
Sadaba, Cruz
Carrillo, Eugenia
Izquierdo, Fernando
del Castillo, José Manuel Benítez
Hurtado, Carolina
del Aguila, Carmen
Fenoy, Soledad
author_facet Magnet, Angela
Gomes, Thiago Santos
Pardinas, Carmen
Garcia de Blas, Natalia
Sadaba, Cruz
Carrillo, Eugenia
Izquierdo, Fernando
del Castillo, José Manuel Benítez
Hurtado, Carolina
del Aguila, Carmen
Fenoy, Soledad
author_sort Magnet, Angela
collection PubMed
description BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. METHODS: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. RESULTS: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. CONCLUSIONS: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out.
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spelling pubmed-57787932018-01-31 Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach Magnet, Angela Gomes, Thiago Santos Pardinas, Carmen Garcia de Blas, Natalia Sadaba, Cruz Carrillo, Eugenia Izquierdo, Fernando del Castillo, José Manuel Benítez Hurtado, Carolina del Aguila, Carmen Fenoy, Soledad Parasit Vectors Research BACKGROUND: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. METHODS: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. RESULTS: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. CONCLUSIONS: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out. BioMed Central 2018-01-22 /pmc/articles/PMC5778793/ /pubmed/29357901 http://dx.doi.org/10.1186/s13071-018-2639-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Magnet, Angela
Gomes, Thiago Santos
Pardinas, Carmen
Garcia de Blas, Natalia
Sadaba, Cruz
Carrillo, Eugenia
Izquierdo, Fernando
del Castillo, José Manuel Benítez
Hurtado, Carolina
del Aguila, Carmen
Fenoy, Soledad
Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
title Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
title_full Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
title_fullStr Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
title_full_unstemmed Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
title_short Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach
title_sort can artificial tears prevent acanthamoeba keratitis? an in vitro approach
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778793/
https://www.ncbi.nlm.nih.gov/pubmed/29357901
http://dx.doi.org/10.1186/s13071-018-2639-5
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