Cargando…
ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition
In animal embryos, control of development is passed from exclusively maternal gene products to those encoded by the embryonic genome in a process referred to as the maternal-to-zygotic transition (MZT). We show that the RNA-binding protein, ME31B, binds to and represses the expression of thousands o...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779226/ https://www.ncbi.nlm.nih.gov/pubmed/28875934 http://dx.doi.org/10.7554/eLife.27891 |
_version_ | 1783294497816313856 |
---|---|
author | Wang, Miranda Ly, Michael Lugowski, Andrew Laver, John D Lipshitz, Howard D Smibert, Craig A Rissland, Olivia S |
author_facet | Wang, Miranda Ly, Michael Lugowski, Andrew Laver, John D Lipshitz, Howard D Smibert, Craig A Rissland, Olivia S |
author_sort | Wang, Miranda |
collection | PubMed |
description | In animal embryos, control of development is passed from exclusively maternal gene products to those encoded by the embryonic genome in a process referred to as the maternal-to-zygotic transition (MZT). We show that the RNA-binding protein, ME31B, binds to and represses the expression of thousands of maternal mRNAs during the Drosophila MZT. However, ME31B carries out repression in different ways during different phases of the MZT. Early, it represses translation while, later, its binding leads to mRNA destruction, most likely as a consequence of translational repression in the context of robust mRNA decay. In a process dependent on the PNG kinase, levels of ME31B and its partners, Cup and Trailer Hitch (TRAL), decrease by over 10-fold during the MZT, leading to a change in the composition of mRNA–protein complexes. We propose that ME31B is a global repressor whose regulatory impact changes based on its biological context. |
format | Online Article Text |
id | pubmed-5779226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-57792262018-01-25 ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition Wang, Miranda Ly, Michael Lugowski, Andrew Laver, John D Lipshitz, Howard D Smibert, Craig A Rissland, Olivia S eLife Developmental Biology In animal embryos, control of development is passed from exclusively maternal gene products to those encoded by the embryonic genome in a process referred to as the maternal-to-zygotic transition (MZT). We show that the RNA-binding protein, ME31B, binds to and represses the expression of thousands of maternal mRNAs during the Drosophila MZT. However, ME31B carries out repression in different ways during different phases of the MZT. Early, it represses translation while, later, its binding leads to mRNA destruction, most likely as a consequence of translational repression in the context of robust mRNA decay. In a process dependent on the PNG kinase, levels of ME31B and its partners, Cup and Trailer Hitch (TRAL), decrease by over 10-fold during the MZT, leading to a change in the composition of mRNA–protein complexes. We propose that ME31B is a global repressor whose regulatory impact changes based on its biological context. eLife Sciences Publications, Ltd 2017-09-06 /pmc/articles/PMC5779226/ /pubmed/28875934 http://dx.doi.org/10.7554/eLife.27891 Text en © 2017, Wang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Wang, Miranda Ly, Michael Lugowski, Andrew Laver, John D Lipshitz, Howard D Smibert, Craig A Rissland, Olivia S ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition |
title | ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition |
title_full | ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition |
title_fullStr | ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition |
title_full_unstemmed | ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition |
title_short | ME31B globally represses maternal mRNAs by two distinct mechanisms during the Drosophila maternal-to-zygotic transition |
title_sort | me31b globally represses maternal mrnas by two distinct mechanisms during the drosophila maternal-to-zygotic transition |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5779226/ https://www.ncbi.nlm.nih.gov/pubmed/28875934 http://dx.doi.org/10.7554/eLife.27891 |
work_keys_str_mv | AT wangmiranda me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition AT lymichael me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition AT lugowskiandrew me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition AT laverjohnd me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition AT lipshitzhowardd me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition AT smibertcraiga me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition AT risslandolivias me31bgloballyrepressesmaternalmrnasbytwodistinctmechanismsduringthedrosophilamaternaltozygotictransition |